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Series GSE197256 Query DataSets for GSE197256
Status Public on Feb 26, 2022
Title Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A single H4 variant gene (H4r) that is located outside of the histone cluster and gives rise to a polyA tailed messager RNA via replication-independent expression is preserved in Drosophila strains despite that its protein product is identical with canonical H4. In order to reveal information on the possible role of this alternative H4 we epitope tagged endogenous H4r and revealed its genome-wide localization to chromatin at the nucleosomal level. Comparing H4r-localization to the localization of another histone variant H3.3 we found that the genome-wide distribution of H4r is more similar to the histone variant H3.3 than to the canonical histone H3. Our ChIP-seq experiments revealed H4r association with regulatory regions of genes involved in cellular stress response. The data presented here indicate that H4r has a variant histone function.
Overall design Examination of the genome-wide localization of two epitope-tagged variant histones at nucleosomal level via ChIP-seq.
Contributor(s) Abraham A, Boros IM, Henn L, Villanyi Z, Zsindely N, Bodai L, Nagy G, Szabo A
Citation(s) 35322122
BioProject PRJNA789790
Submission date Feb 23, 2022
Last update date Mar 25, 2022
Contact name Andrea Ábrahám
Organization name University of Szeged
Street address Közép fasor 52.
City Szeged
State/province Csongrád
ZIP/Postal code 6726
Country Hungary
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (24)
GSM5911648 H4r-rep1-NC-FLAGChIP
GSM5911649 H4r-rep2-NC-FLAGChIP
GSM5911650 H4r-rep1-HS-FLAGChIP

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE197256_RAW.tar 323.2 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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