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Series GSE197159 Query DataSets for GSE197159
Status Public on May 06, 2024
Title Searching for new molecules involved in Anopheles mosquitoes’ response to Plasmodium infection
Organism Anopheles stephensi
Experiment type Expression profiling by high throughput sequencing
Summary Plasmodium parasites within mosquitoes are exposed to various physiological processes, such as blood meal digestion activity, the gonotrophic cycle, and host responses preventing the entry of parasites into the midgut wall. However, when in vitro-cultured ookinetes are injected into the hemocoel of mosquitoes, Plasmodium parasites are not affected by the vertebrate host’s blood contents and do not pass through the midgut epithelial cells. This infection method might aid in identifying mosquito-derived factors affecting Plasmodium development within mosquitoes. This study investigated novel mosquito-derived molecules related to parasite development in Anopheles mosquitoes. We injected in vitro-cultured Plasmodium berghei (ANKA strain) ookinetes into female and male Anopheles stephensi (STE2 strain) mosquitoes and found that the oocyst number was significantly higher in males than in females, suggesting that male mosquitoes better support the development of parasites. Next, RNA-seq analysis was performed on the injected female and male mosquitoes to identify genes exhibiting changes in expression. Five genes with different expression patterns between sexes and greatest expression changes were identified as being potentially associated with Plasmodium infection. Two of the five genes also showed expression changes with infection by blood-feeding, indicating that these genes could affect the development of Plasmodium parasites in mosquitoes.
 
Overall design Male and female mosquitoes were analyzed 24 h & 72 h post injection with P. berghei ookinetes or with ookinete medium (negative control).
 
Contributor(s) Haraguchi A, Takano M, Fujiwara K, Hakozaki J, Nakayama K, Nakamura S, Yoshikawa Y, Fukumoto S, Kusakisako K, Ikadai H
Citation(s) 38569883
Submission date Feb 22, 2022
Last update date May 06, 2024
Contact name Asako Haraguchi
E-mail(s) dv21005@st.kitasato-u.ac.jp
Organization name Kitasato University
Department School of Veterinary Medicine
Lab Veterinary Parasitology
Street address Higashi-23-35-1
City Towada
State/province Aomori
ZIP/Postal code 034-8628
Country Japan
 
Platforms (1)
GPL31974 Illumina NovaSeq 6000 (Anopheles stephensi)
Samples (8)
GSM5910452 24hr Female Injection
GSM5910453 24hr Female Negative
GSM5910454 24hr Male Injection
Relations
BioProject PRJNA809222

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Supplementary file Size Download File type/resource
GSE197159_Expression_Profile.Anopheles-stephensi-Indian_SCAFFOLDS_AsteI2.gene_24h.txt.gz 451.6 Kb (ftp)(http) TXT
GSE197159_Expression_Profile.Anopheles-stephensi-Indian_SCAFFOLDS_AsteI2.gene_72h.txt.gz 449.5 Kb (ftp)(http) TXT
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