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Series GSE197064 Query DataSets for GSE197064
Status Public on Dec 21, 2022
Title Single cell transcriptomic and spatial landscapes of the developing human pancreas
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The progress made in directed differentiation of stem cells has shown that understanding human pancreas development can provide cues for generating unlimited amounts of insulin-producing beta cells for transplantation therapy in diabetes. However, current differentiation protocols have not been successful in reproducibly generating functional human beta cells in vitro, partly due to incomplete understanding of human pancreas development. Here, we present detailed transcriptomic analysis of the various cell types of the developing human pancreas, including their spatial gene patterns. We integrated single cell RNA sequencing with spatial transcriptomics at multiple developmental timepoints and revealed distinct temporal-spatial gene cascades in the developing human pancreas.
Overall design Human fetal pancreases at 12, 13, 14, 15, 18, 19 and 20 post-conception weeks were subject to single cell RNA-seq analysis
Web link
Contributor(s) Olaniru OE, Kadolsky U, Kannambath S, Vaikkinen H, Fung K, Dhami P, Persaud S
Citation(s) 36513063
Submission date Feb 20, 2022
Last update date Dec 24, 2022
Contact name Oladapo Edward Olaniru
Organization name King's College London
Department Department of Diabetes
Lab Shanta Persuad Lab
Street address Hodgkin building, Guy's campus
City London
ZIP/Postal code SE1 1UL
Country United Kingdom
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (8)
GSM5907965 fetal pancreas pool_1_121313_PCW_Gene expression
GSM5907966 fetal pancreas pool_2_121419_PCW_Gene expression
GSM5907967 fetal pancreas pool_3_131419_PCW_Gene expression
BioProject PRJNA808751

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GSE197064_RAW.tar 504.2 Mb (http)(custom) TAR (of CSV)
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