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| Status |
Public on Feb 23, 2023 |
| Title |
p53 ChIP-seq of primary astrocytes in vitro |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate the transcriptional regulation by p53 in astrocytes, we performed ChIP-sequencing using p53 antibody (Leica CM-5) or rabbit IgG control in wildtype primary mouse cortical astrocytes.
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| Overall design |
Primary astrocytes from a total of 8 P3 mouse brains were cultured in the presence of BMP4. ChIP was performed using the EZ-Magna ChIP kit (Millipore) according to manufacturers instructions.
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| Web link |
https://doi.org/10.1016/j.cub.2023.02.013
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| Contributor(s) |
Parrinello S, Marguerat S |
| Citation(s) |
36841240 |
| |
| Submission date |
Feb 16, 2022 |
| Last update date |
Mar 28, 2023 |
| Contact name |
Simona Parrinello |
| Organization name |
UCL Cancer Institute
|
| Street address |
72 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6DD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA807631 |
