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| Status |
Public on May 26, 2022 |
| Title |
Newly recruited intraepithelial Ly6A+CCR9+CD4+ T cells protect against enteric viral infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The intestinal epithelium comprises the body’s largest surface exposed to viruses. Additionally, the gut epithelium hosts a large population of intraepithelial T lymphocytes, or IELs, although their role in resistance against viral infections remain elusive. By fate-mapping T cells recruited to the murine intestine, we observed accumulation of newly recruited CD4+ T cells after infection with murine norovirus CR6 and adenovirus type-2 (AdV), but not reovirus. CR6 and AdV-recruited intraepithelial CD4+ T cells co-express Ly6A and CCR9, exhibit T helper 1 and cytotoxic profiles and confer protection against AdV in vivo and in an organoid model in an IFN-g-dependent manner. Ablation of the T cell receptor (TCR) or the transcription factor ThPOK in CD4+ T cells prior to AdV infection prevented viral control, while TCR ablation during infection did not impact viral clearance. These results uncover a protective role for intraepithelial Ly6A+CCR9+CD4+ T cells against enteric adenovirus.
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| Overall design |
RNAseq analysis of recently recruited small intestinal intraepithelial CD4+CD62L-Tomato+ T cells 10 days post murine Adenovirus-2 infected or PBS treated mice.
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| Contributor(s) |
Parsa R, London M, Rezende de Castro TB, Reis B, Buissant des Amorie J, Smith JG, Mucida D |
| Citation(s) |
35617965 |
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| Submission date |
Feb 09, 2022 |
| Last update date |
Aug 25, 2022 |
| Contact name |
Roham Parsa |
| E-mail(s) |
Rohamparsa@gmail.com
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| Phone |
2123277521
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| Organization name |
Rockefeller University
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| Lab |
Mucosal Immunology
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| Street address |
1230 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA804864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196417_RAW.tar |
20.4 Mb |
(http)(custom) |
TAR (of CSV, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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