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| Status |
Public on Jun 28, 2023 |
| Title |
Gene expression in the mTSCs of WT and Pr-set7 KO mTSCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To detect the gene profiles in WT and Pr-set7 KO mTSCs, mTSCs are collected and subjected to RNA-Seq. After aligned to mouse mm10 by HISAT2, RPKM value was calculated by Edger. Our results show that Pr-set7 as the key regulator for mTSCs. There are also some genes differentially expressed after Pr-set7 KO, some of the DEGs were further confirmed by qPCR, the DEGs were associated with viral mimic inflammatory activities, antiviral innate immune response, genomic instability, and programmed cell death, indicating the essential role of Pr-set7. This RNA-Seq data provides fundamental information for our further physiological study of Pr-set7.
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| Overall design |
Total RNAs in mTSCs from two independent experiments were extracted by TRIzol and subjected to RNA-Seq by MGISEQ 2000. The expression of level of each gene is normalized to RPKM.
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| Contributor(s) |
Lu J |
| Citation(s) |
37307441 |
| |
| Submission date |
Feb 06, 2022 |
| Last update date |
Jun 28, 2023 |
| Contact name |
Jinhua Lu |
| E-mail(s) |
jinhua888@126.com
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| Organization name |
Xiamen University
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| Street address |
No. 4221, Xiangan Road, Xiangan District, Xiamen city
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| City |
Xiamen |
| ZIP/Postal code |
361000 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA803920 |
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