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Status |
Public on Feb 07, 2022 |
Title |
RNA-Seq of inflammatory stimulated HUVECs transfected with microRNA-125a-5p or negative control. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: In recent years, research has revealed the role of microRNAs (miRs) as important regulators of endothelial function. Notably, miR-125a is upregulated in the blood of patients with acute vascular diseases. Since miRNAs act out their function in networks, we aimed at investigating the presence of a miR-125a-related network regulating the endothelial barrier. Method: We investigated transcriptional changes of human umbilical cord endothelial cells (HUVEC) after miR-125a overexpression in an acute inflammatory in-vitro setting using Next Generation Sequencing. Briefly, primary HUVECs from five different donors were transfected either with hsa-miR-125a-5p or negative control (NC). After transfection HUVECs were cultivated for 18 hours and stimulated with TNF (25 ng/ml) for additional 6 hours. We compared the following groups: NC (18047-0001, 18047-0002, 18047-0003, 18047-0004, 18047-0005) versus miR125a: (18047-0006, 18047-0007, 18047-0008, 18047-0009, 18047-0010) Results: MiR-125a overexpression in inflammatory activated HUVECs significantly altered gene expression of 468 genes (FC: +1.25 to -1.25 p<0.01). We found eight potential miR-125a direct target genes involved in endothelial barrier function.
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Overall design |
Transcriptome anlysis via Next Generation Sequencing of HUVECs overexpressing miR-125a-5p (n=5) versus control miRNA (n=5).
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Contributor(s) |
Mueller MB, Liesske V, Li L, Simone K |
Citation(s) |
35401562 |
BioProject |
PRJNA784113 |
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Submission date |
Feb 04, 2022 |
Last update date |
Apr 19, 2022 |
Contact name |
Martin Bernhard Mueller |
E-mail(s) |
Martin_Bernhard.Mueller@med.uni-muenchen.de
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Organization name |
University Hospital, Ludwig-Maximilians-University Munich (LMU)
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Department |
Department of Anaesthesiology
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Lab |
Walter Brendel Center of Experimental Medicine (WBex)
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Street address |
Marchioninistrasse 15
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City |
Munich |
ZIP/Postal code |
81377 |
Country |
Germany |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (10)
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