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| Status |
Public on Jul 19, 2023 |
| Title |
An engineered Sox17 induces somatic to neural stem cell fate transition independently from pluripotency reprogramming [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Advances in molecular strategies to reprogram cell fate is a promising avenue for cell-based therapies. Methods to generate induced neural stem cells (iNSCs) are often slow with limited reprogramming events and it is unclear whether cells transit via a pluripotent state. We report an iNSC reprogramming approach from embryonic and aged mouse fibroblasts using an engineered Sox17 (eSox17FNV). eSox17FNV efficiently drives iNSC reprogramming while Sox2 or Sox17 fails to do so. eSox17FNV acquires the capacity to bind new protein partners to scan the genome more efficiently and possesses a more potent transactivation domain than Sox2. At the onset of reprogramming, in the presence of eSox17FNV, fibroblasts divert from a iPSC route towards iNSC fate. Further, lineage tracing shows that emerging iNSCs do not transit through a pluripotent state if POU factors are excluded. This reveals new molecular and physiological framework for iNSC generation and contrasts with lineage from pluripotency reprogramming.
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| Overall design |
We analyzed the gene expression profiles of Sox2, Sox17 and Sox17FNV mediated somatic cell reprogramming towards iNSCs in NSC medium at different reprogramming time points (Days 4, 8, 12, 18, 24, 30) by RNA-seq. In addition, we also sequenced a Sox17FNV mediated iNSC and iPSC reprogramming in parallel at day 4, 8, 12, 16, 20, 24 in chemically defined medium.
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| Contributor(s) |
Weng M, Jauch R |
| Citation(s) |
37611093 |
| |
| Submission date |
Jan 27, 2022 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Mingxi WENG |
| E-mail(s) |
mingxi@connect.hku.hk
|
| Phone |
55326522
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| Organization name |
School of Biomedical Sciences, The University of Hong Kong
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| Street address |
L408A, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong
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| City |
Hong Kong |
| State/province |
Not Applicable |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (74)
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| This SubSeries is part of SuperSeries: |
| GSE195556 |
An engineered Sox17 induces somatic to neural stem cell fate transition independently from pluripotency reprogramming |
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| Relations |
| BioProject |
PRJNA801242 |
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