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Series GSE194003 Query DataSets for GSE194003
Status Public on Jul 07, 2022
Title Characterization of the crosstalk between Notch signaling and hypoxia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The Notch signaling pathway regulates several differentiation and developmental processes. Reduction of oxygen availability, a condition referred to as hypoxia, impacts on the Notch response. Here we made use of RNA-Seq and ChIP-Seq analysis to investigate the Notch/hypoxia crosstalk at genomic level.
 
Overall design Mouse pre-T cells (Beko) were treated with the DMOG or kept under low oxygen concentration to induce hypoxia. Effects on gene expression were investigated by RNA-Seq and RBPJ occupancy was analyzed by ChIP-Seq.
 
Contributor(s) Friedrich T, Bartkuhn M, Giaimo BD, Ferrante F
Citation(s) 35821235
Submission date Jan 19, 2022
Last update date Jul 20, 2022
Contact name Tobias Friedrich
E-mail(s) tobias.friedrich@pennmedicine.upenn.edu
Organization name University of Pennsylvania
Street address 421 Curie Boulevard
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (30)
GSM5825465 RNA-seq in Beko cells, DMOG_1
GSM5825466 RNA-seq in Beko cells, DMOG_2
GSM5825467 RNA-seq in Beko cells, DMSO_1
Relations
BioProject PRJNA798729

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE194003_RAW.tar 3.0 Gb (http)(custom) TAR (of BW)
GSE194003_RNA_Seq_Counts.xlsx 1.6 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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