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| Status |
Public on Feb 25, 2022 |
| Title |
Single-cell RNA-sequencing of IL-21-tRFP+ GP66+ splenic CD4+ T cells on days 7 and 14 post LCMV Cl13 infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CD4+ T cell-derived interleukin 21 (IL-21) sustains CD8+ T cell responses during chronic viral infection, but the helper subset that confers this protection remains unclear. Here, we applied scRNA and ATAC-seq approaches to determine the heterogeneity of IL-21+CD4+ T cells during LCMV clone 13 infection.
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| Overall design |
IL-21-tRFP+ GP66-specific CD4+ T cells were FACS-sorted from LCMV Cl13-infected IL-21-tRFP 10Bit reporter mice on days 7 and 14 p.i. (with 2 donor mice per time point) and were loaded on the Chromium Controller (10x Genomics). Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ v2 Reagent Kit (10x Genomics) according to manufacturer’s protocol
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| Contributor(s) |
Cui W, Zander R |
| Citation(s) |
35216666 |
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| Submission date |
Jan 18, 2022 |
| Last update date |
Mar 01, 2022 |
| Contact name |
Weiguo Cui |
| E-mail(s) |
wcui04@gmail.com
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| Phone |
312-503-0332
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| Organization name |
Northwestern University
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| Department |
Pathology
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| Lab |
Cui
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| Street address |
303 E Chicago Ave
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60654 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA798421 |
