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Series GSE193215 Query DataSets for GSE193215
Status Public on Jun 23, 2022
Title PHF20 Activates Autophagy Genes through Enhancer Activation via H3K36me2 Binding Activity [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Autophagy is a catabolic pathway that maintains cellular homeostasis under various stress conditions, including nutrient-deprived conditions. To elevate autophagic flux to a sufficient level under stress conditions, transcriptional activation of autophagy genes occurs to replenish autophagy components. Here, using combination of RNA-seq, ATAC-seq and ChIP-seq, we demonstrated found that plant homeodomain finger protein 20 (Phf20PHF20), which is an epigenetic reader possessing methyl binding activity, plays a key role in controlling the expression of autophagy genes. PHF20 activates autophagy genes through enhancer activation via H3K36me2 binding activity as an epigenetic reader and that our findings emphasize the importance of nuclear regulation of autophagy.
Overall design mRNA-seq, ATAC-seq and ChIP-seq experiments under normal and 24hrs of glucose starvation condition in WT and Phf20-/- MEFs
Contributor(s) Park SW, Park D, Baek SH
Citation(s) 35821310
Submission date Jan 07, 2022
Last update date Aug 24, 2022
Contact name Se Won Park
Organization name Seoul national university
Street address Gwanak-ro 1, Gwanak-gu, Seoul, Seoul Nat'l Univ Bldg504 Rm607
City Seoul
State/province Gwanak-Gu
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM5776726 WT, control, ATAC-seq, rep1
GSM5776727 WT, control, ATAC-seq, rep2
GSM5776728 WT, GlcStarv, ATAC-seq, rep1
This SubSeries is part of SuperSeries:
GSE193393 PHF20 Activates Autophagy Genes through Enhancer Activation via H3K36me2 Binding Activity
BioProject PRJNA795443

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Supplementary file Size Download File type/resource
GSE193215_ATACseq_KO-CTL.merged.dedup.bigwig 295.3 Mb (ftp)(http) BIGWIG
GSE193215_ATACseq_KO-GS.merged.dedup.bigwig 227.1 Mb (ftp)(http) BIGWIG
GSE193215_ATACseq_WT-CTL.merged.dedup.bigwig 254.5 Mb (ftp)(http) BIGWIG
GSE193215_ATACseq_WT-GS.merged.dedup.bigwig 189.1 Mb (ftp)(http) BIGWIG
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