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Series GSE193176 Query DataSets for GSE193176
Status Public on Feb 02, 2023
Title Environmentally relevant exposure to TBBPA and its analogues may not drastically affect human early cardiac development
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Tetrabromobisphenol A (TBBPA) and its substitutes and derivatives have been widely used as halogenated flame retardants (HFRs), in the past few decades. As a consequence, these compounds are frequently detected in the environment, as well as human bodily fluids, especially umbilical cord blood and breast milk. This has raised awareness of their potential risks to fetuses and infants. In this study, we employed human embryonic stem cell differentiation models to assess the potential developmental toxicity of six TBBPA-like compounds, at human relevant nanomolar concentrations. To mimic early embryonic development, we utilized embryoid body-based 3D differentiation in presence of the six HFRs. Transcriptomics data showed that HFR exposure over 16 days of differentiation only interfered with the expression of a few genes, indicating those six HFRs may not have specific tissue/organ targets during embryonic development. Nevertheless, further analyses revealed that some cardiac-related genes were dysregulated. Since the heart is also the first organ to develop, we employed a cardiac differentiation model to analyze the six HFRs' potential developmental toxicity in more depth. Overall, HFRs of interest did not significantly disturb the canonical WNT pathway, which is an essential signal transduction pathway for cardiac development. In addition, the six HFRs showed only mild changes in gene expression levels for cardiomyocyte markers, such as NKX2.5, MYH7, and MYL4, as well as a significant down-regulation of some but not all the epicardial and smooth muscle cell markers selected. Taken together, our results show that the six studied HFRs, at human relevant concentrations, may impose negligible effects on embryogenesis and heart development. Nevertheless, higher exposure doses might affect the early stages of heart development.
 
Overall design The differences of RNA expression in embryoid bodies after exposure to different compounds were examined.
 
Contributor(s) Zhao M, Yin N, Yang R, Li S, Zhang S, Faiola F
Citation(s) 35577262
Submission date Jan 06, 2022
Last update date May 04, 2023
Contact name Renjun Yang
E-mail(s) rjyang@rcees.ac.cn
Phone 18811781909
Organization name RCEES
Street address 双清路18号林业大学北门
City 北京市
State/province 北京
ZIP/Postal code 100085
Country China
 
Platforms (1)
GPL28038 DNBSEQ-G400 (Homo sapiens)
Samples (42)
GSM5775967 hEB_D2_BAE1
GSM5775968 hEB_D2_BAE2
GSM5775969 hEB_D2_BDBPE1
Relations
BioProject PRJNA795182

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193176_core_table_gene.txt.gz 123.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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