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| Status |
Public on Aug 03, 2022 |
| Title |
Transcriptional regulation of the thymus master regulator Foxn1 [RNAseq_AR3_vs_nude] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
FOXN1 is a transcription factor critical for the development of both thymic epithelial cell (TEC) and hair follicle cell (HFC) compartments. However, mechanisms controlling its expression remain poorly understood. To address this question, we performed thorough analyses of the conservation and chromatin status of the Foxn1 locus in different tissues and states, and identified several putative cis-regulatory regions unique to TEC vs. HFC. Furthermore, experiments using genetically modified mice with specific deletions in the Foxn1 locus and additional bioinformatic analyses helped us identify key regions and transcription factors involved in either positive or negative regulation of Foxn1 in both TEC and HFC. Specifically, we identified SIX1 and FOXN1 itself, as key factors inducing Foxn1 expression in embryonic and neonatal TECs. Together, our data provide important mechanistic insights into the transcriptional regulation of the Foxn1 gene in TEC vs. HFC and highlight the role of FOXN1 in its autoregulation
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| Overall design |
mRNA profiles of sorted total thymic epithelial cells (TEC) from young (4-5 weeks old) of Foxn1 del AR3 mutant and heterozygous control (generated in this study) as well as of nu/nu,were generated by deep sequencing, using the MARSseq method.
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| Contributor(s) |
Abramson J, Kadouri N |
| Citation(s) |
36026441 |
| |
| Submission date |
Jan 01, 2022 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Jakub Abramson |
| E-mail(s) |
jakub.abramson@weizmann.ac.il
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| Organization name |
Weizmann Institute of Science
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| Department |
Department of Immunology
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| Street address |
234 Herzl st.
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE192875 |
Transcriptional regulation of the thymus master regulator Foxn1 |
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| Relations |
| BioProject |
PRJNA793578 |
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