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Series GSE192713 Query DataSets for GSE192713
Status Public on Dec 21, 2022
Title Successful Sulphide-driven Partial Denitrification: efficiency, stability and resiliency in SRT-controlled conditions
Organism sulfo-oxidizing enrichment culture
Experiment type Other
Summary The aim of the present work was to maximise NO2- conversion over NO3- reduction coupled with complete sulphide (HS-) oxidation, by the selective control of influent S/N ratio and sludge retention time (SRT). A 2.5-L chemostat was operated for 115 days and three operational phases were tested at decreasing SRT of 40, 23 and 13 h, testing S/N ratios in the range of 0.5-1 gS/gN. Successful sulphide-driven PAD was achieved and lead to average NO2- conversion efficiencies as high as 77±17% at all the conditions tested, with the highest value of 99% at the lowest S/N of 0.58 gS/gN and SRT of 23 h. Respirometric tests showed that NO3- uptake rate was stable at 90±10 mgN/gVSS/h, either when NO3- was present as sole electron acceptor or at NO2- levels as high as 120 mgN/l; on the contrary, NO2- uptake rates were very sensitive to the applied conditions. Metabarcoding analyses revealed that the microbial community was highly enriched in Sulphur Oxidizing Bacteria (SOB>80%) and strict and stable S-limiting conditions appeared to favour Thiobacillus over Sulfurimonas genus. A preliminary assessment of N2O potential emission was also performed. To the best of our knowledge, this is the first work evaluating the synergic effect of SRT and influent S/N ratio on nitrite accumulation in highly SOB-enriched systems and the NO2- conversion efficiencies achieved are among the highest reported in literature.
Overall design Lab-scale chemostat seeded with enriched Sulphur Oxidizing Biomass and fed with sythetic mineral medium rich in sulphide and nitrate
Contributor(s) Polizzi C, Gabriel D, Munz G
Citation(s) 35149015
Submission date Dec 28, 2021
Last update date Dec 21, 2022
Contact name Matteo Ramazzotti
Organization name University of Florence
Department Experimental and Clinical Biomedical Sciences
Street address viale Morgagni 50
City Firenze
ZIP/Postal code 50134
Country Italy
Platforms (1)
GPL31153 Illumina MiSeq (sulfo-oxidizing enrichment culture)
Samples (4)
GSM5761577 1CP
GSM5761578 2CP
GSM5761579 3CP
BioProject PRJNA792821

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Supplementary file Size Download File type/resource
GSE192713_data.xls.gz 64.3 Kb (ftp)(http) XLS
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Processed data are available on Series record

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