|
Status |
Public on Dec 21, 2022 |
Title |
Successful Sulphide-driven Partial Denitrification: efficiency, stability and resiliency in SRT-controlled conditions |
Organism |
sulfo-oxidizing enrichment culture |
Experiment type |
Other
|
Summary |
The aim of the present work was to maximise NO2- conversion over NO3- reduction coupled with complete sulphide (HS-) oxidation, by the selective control of influent S/N ratio and sludge retention time (SRT). A 2.5-L chemostat was operated for 115 days and three operational phases were tested at decreasing SRT of 40, 23 and 13 h, testing S/N ratios in the range of 0.5-1 gS/gN. Successful sulphide-driven PAD was achieved and lead to average NO2- conversion efficiencies as high as 77±17% at all the conditions tested, with the highest value of 99% at the lowest S/N of 0.58 gS/gN and SRT of 23 h. Respirometric tests showed that NO3- uptake rate was stable at 90±10 mgN/gVSS/h, either when NO3- was present as sole electron acceptor or at NO2- levels as high as 120 mgN/l; on the contrary, NO2- uptake rates were very sensitive to the applied conditions. Metabarcoding analyses revealed that the microbial community was highly enriched in Sulphur Oxidizing Bacteria (SOB>80%) and strict and stable S-limiting conditions appeared to favour Thiobacillus over Sulfurimonas genus. A preliminary assessment of N2O potential emission was also performed. To the best of our knowledge, this is the first work evaluating the synergic effect of SRT and influent S/N ratio on nitrite accumulation in highly SOB-enriched systems and the NO2- conversion efficiencies achieved are among the highest reported in literature.
|
|
|
Overall design |
Lab-scale chemostat seeded with enriched Sulphur Oxidizing Biomass and fed with sythetic mineral medium rich in sulphide and nitrate
|
|
|
Contributor(s) |
Polizzi C, Gabriel D, Munz G |
Citation(s) |
35149015 |
|
Submission date |
Dec 28, 2021 |
Last update date |
Dec 21, 2022 |
Contact name |
Matteo Ramazzotti |
E-mail(s) |
matteo.ramazzotti@unifi.it
|
Organization name |
University of Florence
|
Department |
Experimental and Clinical Biomedical Sciences
|
Street address |
viale Morgagni 50
|
City |
Firenze |
ZIP/Postal code |
50134 |
Country |
Italy |
|
|
Platforms (1) |
GPL31153 |
Illumina MiSeq (sulfo-oxidizing enrichment culture) |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA792821 |