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Status |
Public on Dec 31, 2023 |
Title |
Identification of TBX3 and shared HAND2-TBX3 target gene in early mouse limb buds [RNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
ChIP-seq is combined with RNA-seq analysis to identify the TBX3 and HAND2 target genes during mouse limb bud development. This analysis identifies the DEGs and direct transcriptional targets of HAND2 and TBX3 during the early determinative period critical to establishment of limb axis polarity and the SHH signaling center in the posterior limb bud mesenchyme. In particular, bioinformatics analysis identifies the target gene networks that are co-regulated by both TBX3 and HAND2 transcription factors in the limb bud mesenchyme. A significant fraction of the target genes identified are required for normal limb bud development and their spatio-temporal expression patterns are changed in mutant limb buds at early stages. This project was supported by SNSF Grant 310030_184734 to Rolf Zeller (project partner: Aimée Zuniga).
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Overall design |
RNA-seq analysis of a) Hand2-deficient and b) Tbx3-deficient mouse forelimb buds
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Contributor(s) |
Jhanwar S, Girdziusaite A, Zuniga A, Zeller R |
Citation missing |
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Submission date |
Dec 22, 2021 |
Last update date |
Dec 31, 2023 |
Contact name |
Aimee Zuniga |
E-mail(s) |
devgenlab-dbm@unibas.ch
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Organization name |
University of Basel
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Department |
Department of Biomedicine
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Lab |
Developmental Genetics
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Street address |
Mattenstrasse, 28 4058
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City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (12)
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GSM5747751 |
RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 1 |
GSM5747752 |
RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 2 |
GSM5747753 |
RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 3 |
GSM5747754 |
RNA-seq FL bud E9.75-E10 Tbx3_del replicate 1 |
GSM5747755 |
RNA-seq FL bud E9.75-E10 Tbx3_del replicate 2 |
GSM5747756 |
RNA-seq FL bud E9.75-E10 Tbx3_del replicate 3 |
GSM5747757 |
RNA-seq FL bud E10-E10.25 Hand2_WT replicate 1 |
GSM5747758 |
RNA-seq FL bud E10-E10.25 Hand2_WT replicate 2 |
GSM5747759 |
RNA-seq FL bud E10-E10.25 Hand2_WT replicate 3 |
GSM5747760 |
RNA-seq FL bud E10-E10.25 Hand2_del replicate 1 |
GSM5747761 |
RNA-seq FL bud E10-E10.25 Hand2_del replicate 2 |
GSM5747762 |
RNA-seq FL bud E10-E10.25 Hand2_del replicate 3 |
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This SubSeries is part of SuperSeries: |
GSE192486 |
Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds |
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Relations |
BioProject |
PRJNA791648 |