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Series GSE192485 Query DataSets for GSE192485
Status Public on Dec 31, 2023
Title Identification of TBX3 and shared HAND2-TBX3 target gene in early mouse limb buds [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary ChIP-seq is combined with RNA-seq analysis to identify the TBX3 and HAND2 target genes during mouse limb bud development. This analysis identifies the DEGs and direct transcriptional targets of HAND2 and TBX3 during the early determinative period critical to establishment of limb axis polarity and the SHH signaling center in the posterior limb bud mesenchyme. In particular, bioinformatics analysis identifies the target gene networks that are co-regulated by both TBX3 and HAND2 transcription factors in the limb bud mesenchyme. A significant fraction of the target genes identified are required for normal limb bud development and their spatio-temporal expression patterns are changed in mutant limb buds at early stages. This project was supported by SNSF Grant 310030_184734 to Rolf Zeller (project partner: Aimée Zuniga).
 
Overall design RNA-seq analysis of a) Hand2-deficient and b) Tbx3-deficient mouse forelimb buds
 
Contributor(s) Jhanwar S, Girdziusaite A, Zuniga A, Zeller R
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Submission date Dec 22, 2021
Last update date Dec 31, 2023
Contact name Aimee Zuniga
E-mail(s) devgenlab-dbm@unibas.ch
Organization name University of Basel
Department Department of Biomedicine
Lab Developmental Genetics
Street address Mattenstrasse, 28 4058
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM5747751 RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 1
GSM5747752 RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 2
GSM5747753 RNA-seq FL bud E9.75-E10 Tbx3_WT replicate 3
This SubSeries is part of SuperSeries:
GSE192486 Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds
Relations
BioProject PRJNA791648

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE192485_RAW.tar 2.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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