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Series GSE192484 Query DataSets for GSE192484
Status Public on Dec 31, 2023
Title Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ChIP-seq is combined with RNA-seq analysis to identify the TBX3 and HAND2 target genes during mouse limb bud development. This analysis identifies the DEGs and direct transcriptional targets of HAND2 and TBX3 during the early determinative period critical to establishment of limb axis polarity and the SHH signaling center in the posterior limb bud mesenchyme. In particular, bioinformatics analysis identifies the target gene networks that are co-regulated by both TBX3 and HAND2 transcription factors in the limb bud mesenchyme. A significant fraction of the target genes identified are required for normal limb bud development and their spatio-temporal expression patterns are changed in mutant limb buds at early stages. This project was supported by SNSF Grant 310030_184734 to Rolf Zeller (project partner: Aimée Zuniga).
Overall design Identification of the genomic regions enriched in TBX3 chromatin complexes in mouse limb buds using ChIP-seq
Contributor(s) Jhanwar S, Sheth R, Zuniga A, Zeller R
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Submission date Dec 22, 2021
Last update date Dec 31, 2023
Contact name Aimee Zuniga
Organization name University of Basel
Department Department of Biomedicine
Lab Developmental Genetics
Street address Mattenstrasse, 28 4058
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM5747747 ChIP Tbx3 FL bud E9.75-E10.25 replicate 1
GSM5747748 Input Tbx3 FL bud E9.75-E10.25 replicate 1
GSM5747749 ChIP Tbx3 FL bud E9.75-E10.25 replicate 2
This SubSeries is part of SuperSeries:
GSE192486 Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds
BioProject PRJNA791649

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE192484_RAW.tar 2.0 Gb (http)(custom) TAR (of BB, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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