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Series GSE192440 Query DataSets for GSE192440
Status Public on Feb 10, 2023
Title RNA m6A methylome in mouse oocytes and preimplantation embryos
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary N6-methyladenosine (m6A), the most prevalent internal modification in eukaryotic messenger RNA, plays diverse regulatory roles in many biological processes and is involved in a variety of physiological behaviors. Here we present the m6A methylome maps of mouse oocytes and preimplantation embryos by a low-input approach picoMeRIP-seq. We revealed that m6A was largely and dynamically deposited on maternal and zygotic RNAs during maternal-to-zygotic transition (MZT), especially on those encoding the factors essential for cell fate determination. m6A frequently marked the maternally-derived RNAs to be degraded and the genes that were activated during zygotic genome activation. Moreover, the RNAs derived from retrotransposons, such as MTA and MERVL, were heavily occupied by m6A. Collectively, our results provide a foundation for future studies exploring the regulatory roles of m6A in mammalian early embryo development.
 
Overall design Using picoMeRIP-seq we developed, the transcriptome-wide m6A maps were generated at six developmental stages including oocytes at germinal vesicle (GV) and metaphase II (MII) stages, and embryos at zygote, 2-cell, 8-cell and blastocyst stages, with two biological replicates for each stage.
 
Contributor(s) Wang Y, Li Y, Klungland A, Dahl JA, Au KF
Citation(s) 37081317
Submission date Dec 21, 2021
Last update date May 12, 2023
Contact name Kin Fai Au
E-mail(s) kinfai@med.umich.edu
Phone 734-615-5510
Organization name University of Michigan
Department Department of Computational Medicine and Bioinformatics
Lab Room 2035, Palmer Commons
Street address 100 Washtenaw Avenue
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM5746910 2-cell, Input, rep1
GSM5746911 2-cell, Input, rep2
GSM5746912 2-cell, IP, rep1
Relations
BioProject PRJNA791403
SRA SRP352000

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Supplementary file Size Download File type/resource
GSE192440_RAW.tar 385.8 Mb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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