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Status |
Public on Feb 10, 2023 |
Title |
RNA m6A methylome in mouse oocytes and preimplantation embryos |
Organism |
Mus musculus |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
N6-methyladenosine (m6A), the most prevalent internal modification in eukaryotic messenger RNA, plays diverse regulatory roles in many biological processes and is involved in a variety of physiological behaviors. Here we present the m6A methylome maps of mouse oocytes and preimplantation embryos by a low-input approach picoMeRIP-seq. We revealed that m6A was largely and dynamically deposited on maternal and zygotic RNAs during maternal-to-zygotic transition (MZT), especially on those encoding the factors essential for cell fate determination. m6A frequently marked the maternally-derived RNAs to be degraded and the genes that were activated during zygotic genome activation. Moreover, the RNAs derived from retrotransposons, such as MTA and MERVL, were heavily occupied by m6A. Collectively, our results provide a foundation for future studies exploring the regulatory roles of m6A in mammalian early embryo development.
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Overall design |
Using picoMeRIP-seq we developed, the transcriptome-wide m6A maps were generated at six developmental stages including oocytes at germinal vesicle (GV) and metaphase II (MII) stages, and embryos at zygote, 2-cell, 8-cell and blastocyst stages, with two biological replicates for each stage.
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Contributor(s) |
Wang Y, Li Y, Klungland A, Dahl JA, Au KF |
Citation(s) |
37081317 |
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Submission date |
Dec 21, 2021 |
Last update date |
May 12, 2023 |
Contact name |
Kin Fai Au |
E-mail(s) |
kinfai@med.umich.edu
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Phone |
734-615-5510
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Organization name |
University of Michigan
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Department |
Department of Computational Medicine and Bioinformatics
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Lab |
Room 2035, Palmer Commons
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Street address |
100 Washtenaw Avenue
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City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (24)
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GSM5746913 |
2-cell, IP, rep2 |
GSM5746914 |
8-cell, Input, rep1 |
GSM5746915 |
8-cell, Input, rep2 |
GSM5746916 |
8-cell, IP, rep1 |
GSM5746917 |
8-cell, IP, rep2 |
GSM5746918 |
Blastocyst, Input, rep1 |
GSM5746919 |
Blastocyst, Input, rep2 |
GSM5746920 |
Blastocyst, IP, rep1 |
GSM5746921 |
Blastocyst, IP, rep2 |
GSM5746922 |
GV oocyte, Input, rep1 |
GSM5746923 |
GV oocyte, Input, rep2 |
GSM5746924 |
GV oocyte, IP, rep1 |
GSM5746925 |
GV oocyte, IP, rep2 |
GSM5746926 |
MII oocyte, Input, rep1 |
GSM5746927 |
MII oocyte, Input, rep2 |
GSM5746928 |
MII oocyte, IP, rep1 |
GSM5746929 |
MII oocyte, IP, rep2 |
GSM5746930 |
Zygote, Input, rep1 |
GSM5746931 |
Zygote, Input, rep2 |
GSM5746932 |
Zygote, IP, rep1 |
GSM5746933 |
Zygote, IP, rep2 |
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Relations |
BioProject |
PRJNA791403 |
SRA |
SRP352000 |
Supplementary file |
Size |
Download |
File type/resource |
GSE192440_RAW.tar |
385.8 Mb |
(http)(custom) |
TAR (of BED, BIGWIG) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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