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| Status |
Public on Dec 31, 2022 |
| Title |
The role of aV Integrin in regulating macrophage phenotype and function during cardiac remodeling after myocardial infarction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages infiltrate the infarcted heart and play a critical role in repair, remodeling and fibrosis. Macrophages sense changes in the extracellular matrix (ECM) environment through Integrins, thus activating signaling pathways that regulate their function. Our data show that av integrin is highly expressed in isolated bone marrow macrophages and is markedly upregulated in response to TGF-β, a growth factor known to be activated in the infarcted heart. Accordingly, we hypothesize that aV integrin induction in infarct macrophages may regulate macrophage phenotype, function and response to key macrophage-activating signals following MI.
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| Overall design |
In order to study the role of integrin aV in mmacrophages in vitro, we isolated bone marrow macrophages from WT mice and grow them in fibronectic coated and normal culture dishes, and treated them with a anti-integrin αV neutralizing antibody and its control (IgG).
***Submitter declares raw data are unavailable and cannot be recovered***
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| Contributor(s) |
Li R, Chen B, Hanna A, Humeres C, Hernandez SC, Li J, Frangogiannis NG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 14, 2021 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Silvia C Hernandez |
| E-mail(s) |
silvia.hernandezvelasco@einsteinmed.edu, silviachernandezv@gmail.com
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| Phone |
9295049664
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| Organization name |
Albert Einstein College of Medicine
|
| Department |
Medicine-Cardiology
|
| Lab |
Frangogiannis
|
| Street address |
1300 Morris Park Avenue
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| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
|
| GSM5733108 |
itgav_1 - BMM + Anti-ITGAV #1 |
| GSM5733109 |
itgav_2 - BMM + Anti-ITGAV #2 |
| GSM5733110 |
itgav_3 - BMM + Anti-ITGAV #3 |
| GSM5733111 |
FN_1 - FN coated dishes + BMM + IggRat #1 |
| GSM5733112 |
FN_1 - FN coated dishes + BMM + IggRat #2 |
| GSM5733113 |
FN_1 - FN coated dishes + BMM + IggRat #3 |
| GSM5733114 |
FN_av_1 - FN coated dishes + BMM + Anti-ITGAV #1 |
| GSM5733115 |
FN_av_2 - FN coated dishes + BMM + Anti-ITGAV #2 |
| GSM5733116 |
FN_av_3 - FN coated dishes + BMM + Anti-ITGAV #3 |
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| Relations |
| BioProject |
PRJNA788769 |
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