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Series GSE190795 Query DataSets for GSE190795
Status Public on Jan 31, 2022
Title IL-9 is required for multi-cytokine producing tissue-resident memory CD4+ T cell-dependent allergic airway recall responses
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Asthma is a chronic inflammatory lung disease with intermittent flares predominately mediated through memory T cells. Yet, the identity of long-term memory cells that mediate allergic recall responses are not well defined. In this report, using a mouse model of chronic allergen exposure followed by an allergen-free rest period, we have characterized a sub-population of Th2 cells that secretes IL-9 as an obligate effector cytokine. IL-9-secreting cells have a resident memory T cell phenotype and blocking IL-9 during a recall challenge significantly diminishes airway inflammation and airway hyperreactivity. T cells secrete IL-9 in response to allergen recall and secretion is amplified by IL-33. Using scRNA-seq and scATAC-seq, we define the cellular identity of a distinct populations of T cells with pro-allergic cytokine patterns. Thus, in a recall model of allergic airway inflammation, IL-9 secretion from a multi-cytokine producing cell population is required for an allergen recall response.
Overall design 1. To compare transcriptional profile of ST2+CD4 T cells and ST2- CD4 T cells in the lung, ST2+ and ST2- CD4 T cells were isolated from the lung in a recall model and pooled together to generate replicates of ST2+ CD4 T cells and ST2- CD4 T cells, with a total of 4 samples for scRNA-seq and snATAC-seq analysis. 2. To examine to the function of IL-9 in allergic recall response, single suspension of total viable cells from the lung were generated from the mice that were treated with anti-IL-9 or isotype (100 μg/ml) during the recall challenge. For this experiment three replicates of total lung cells after anti-IL-9 or isotype treatment were used for scRNA-seq analysis.
Contributor(s) Kaplan MH, Ulrich BJ, Kharwadkar R, Gao H
Citation(s) 35302861
Submission date Dec 13, 2021
Last update date May 02, 2022
Contact name Rakshin Kharwadkar
Organization name Indiana University School of Medicine
Department Microbiology and Immunology
Street address 635 Branhill Dr., 418 MS building
City Indianapolis
State/province Indiana
ZIP/Postal code 46202
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (14)
GSM5732083 ST2_pos_1_scRNA-seq
GSM5732084 ST2_pos_2_scRNA-seq
GSM5732085 ST2_neg_1_scRNA-seq
BioProject PRJNA788490
SRA SRP350543

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Supplementary file Size Download File type/resource
GSE190795_RAW.tar 5.9 Gb (http)(custom) TAR (of CSV, H5, TBI, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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