|
| Status |
Public on Feb 08, 2023 |
| Title |
Lipid-accumulated reactive astrocytes promote seizure activity in epilepsy [Human bulk RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abnormal lipid accumulation have been reported in patients with temporal lobe epilepsy (TLE) by in vivo magnetic resonance imaging (MRI). However, the role of astrocytes in the regulation of neuronal activity or lipid metabolism in epilepsy is unclear. Using single-nucleus RNA sequencing of TLE patient samples, we found lipid accumulation and lipid metabolism dysfunction mainly take place in astrocytes. Mechanistic studies revealed that apolipoprotein E (APOE) mediates lipid transfer from hyperactive neurons to astrocytes, turning them into the neurotoxic reactive phenotype
|
| |
|
| Overall design |
Examination of 3 TLE patents sample and 3 non-epileptic control patients
|
| |
|
| Contributor(s) |
Yan C, Liu X, Chen Z, Fang W, Chen D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 08, 2021 |
| Last update date |
Feb 09, 2023 |
| Contact name |
Dijun Chen |
| E-mail(s) |
dijunchen@nju.edu.cn
|
| Organization name |
Nanjing University
|
| Department |
Life Sciences
|
| Street address |
Xianlin
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
310028 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE190453 |
Lipid-accumulated reactive astrocytes promote seizure activity in epilepsy |
|
| Relations |
| BioProject |
PRJNA787219 |
Less...
More...