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| Status |
Public on Aug 20, 2023 |
| Title |
Bromodomain member BRD4 is required for hematopoietic stem cell functions (CUT&RUN) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate the role of BRD4 in normal hematopoiesis, Brd4 conditional KO mouse model was generated. We purified the Lin-cKit+ cells from bone marrow of WT and Brd4 KO mice and performed the CUT&RUN to study the histone modification and Brd4 occupancy around genome in HSC/HPCs population.
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| Overall design |
Brd4, H3K27Ac, H3K122Ac, H3K4me3, Clipping H3 in WT (n=2) and Brd4 KO (n=2) Lin-Ckit+ cells
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| |
|
| Contributor(s) |
Yang F, Yang H, Sui P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 29, 2021 |
| Last update date |
Aug 21, 2023 |
| Contact name |
Hui Yang |
| Organization name |
UTHSCSA
|
| Street address |
5114 medical dr.
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE189836 |
Bromodomain member BRD4 is required for hematopoietic stem cell functions |
|
| Relations |
| BioProject |
PRJNA784623 |
| SRA |
SRP348494 |
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