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Series GSE189786 Query DataSets for GSE189786
Status Public on Apr 30, 2022
Title Targeted DamID to understand transcription factor genomic occupancy in human foetal lung progenitor cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In order to study the genomic occupancy of transcription factors, including SOX9, ETV4 and ETV5, in human foetal lung tip progenitor cells, we have introduced Targeted DamID system via lentivirus in a human foetal lung tissue derived organoid culture (Nikolic et al., 2017). We were able to identify the corresponding TF motif enrichment and discovered a potential co-regulation function of SOX9 and ETV factors in human feotal lung progenitor cells. This study has pioneered the use of targeted DamID approach in tissue derived organoid system.
Overall design We used 4 different independent organoid lines treated as replicates, all of which are around 8 pcw. We introduced the SOX9 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls. In a following-up experimental setting, we introduced ETV4 and ETV5 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls.
Contributor(s) Sun D, LlorĂ -Batlle O, Van Den Ameele J, Brand AH, Rawlins EL
Citation(s) 36121125
Submission date Nov 29, 2021
Last update date Oct 01, 2022
Contact name Dawei Sun
Organization name University of Cambridge
Department The Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (20)
GSM5707556 SOX9-Dam_rep1
GSM5707557 SOX9-Dam_rep2
GSM5707558 SOX9-Dam_rep3
This SubSeries is part of SuperSeries:
GSE189885 Human fetal lung tip progenitor cells
BioProject PRJNA784520

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 56.9 Mb (ftp)(http) BW
GSE189786_ETV4_25.forGreat.bed.gz 38.9 Kb (ftp)(http) BED 57.1 Mb (ftp)(http) BW
GSE189786_ETV5_50.forGreat.bed.gz 62.6 Kb (ftp)(http) BED
GSE189786_SOX9_50.forGreat.bed.gz 42.8 Kb (ftp)(http) BED 57.7 Mb (ftp)(http) BW
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Processed data are available on Series record

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