NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE18971 Query DataSets for GSE18971
Status Public on Nov 11, 2009
Title Nutlin-3 treatment of B-CLL
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: p53 plays a key role in determining the clinical features of B cell chronic lymphocytic leukemia (CLL). Disruption of p53 by point mutations, deletion at 17p13, or both, occurs in a fraction of cases at diagnosis and predicts poor survival and chemorefractoriness. In cells with functional p53, p53 activity is inhibited through interaction with MDM2. In fact, p53 can be activated upon exposure of cells to inhibitors of p53/MDM2 interaction, like Nutlins. Exposure of CLL cells to Nutlin-3 is effective in raising the levels of p53 protein with subsequent induction of cell cycle arrest and/or apoptosis, independently of the most relevant prognostic markers. Specific gene-sets and GEP were documented to be associated with response or resistance to Nutlin-3 exposure in p53(wt) or p53(del/mut) CLL. These findings may help to identify novel molecular targets for CLL therapy. Aim: to analyze the gene expression profile (GEP) induced by Nutlin-3 exposure in primary CLL cells from p53(wt) and p53(del/mut) cases. Methods: purified cells from 24 PB CLL samples, all characterized for IGHV mutational status, CD38 and ZAP-70 and p53 mutations (16 p53(wt) CLL, 8 p53(del/mut) CLL of which 6 with del17p13 and p53 mutations, 1 with del17p13 alone, and 1 with p53 mutations alone), were exposed to 10 uM Nutlin-3 for 24 hours. GEP was performed using a dual labelling strategy; the differential expression of the below reported genes were validated by quantitative real-time PCR. specific gene-sets and GEP were documented to be associated with response or resistance to Nutlin-3 exposure in p53(wt) or p53(del/mut) CLL. These findings may help to identify novel molecular targets for CLL therapy.
 
Overall design Two-condition experiment, Nutlin-3 Treated B-CLL vs. Untreated B-CLL. Biological replicates: 24 control replicates, 24 Treated replicates. Reference design
 
Contributor(s) Dal Bo M, Secchiero P, Marconi D, Bomben R, Benedetti D, Zucchetto A, Pozzato G, Gaidano G, Del Poeta G, Forconi F, Zauli G, Gattei V
Citation(s) 20507307
Submission date Nov 11, 2009
Last update date Feb 22, 2018
Contact name daniela marconi
E-mail(s) daniela.marconi@gmail.com
Organization name CRO AVIANO
Street address Via franco Gallini 2
City Aviano
ZIP/Postal code 33081
Country Italy
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (48)
GSM469593 Nutlin-3 Treated repl 1
GSM469594 Nutlin-3 Treated repl 2
GSM469595 Nutlin-3 Treated repl 3
Relations
BioProject PRJNA121019

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18971_RAW.tar 642.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap