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Series GSE189443 Query DataSets for GSE189443
Status Public on Jul 05, 2022
Title miRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary DCMU, or diuron, is a widely used herbicide, which can cause adverse effects on human. Here, we develop an approach that combines functional, miRNA and RNA sequencing data to evaluate the effects of DCMU exposure on the human immune system, notably on CD8+ T cells, as the main effectors of the organism’s defense against viruses or neoplastic cells. We found that DCMU reduces T cell abilities, in vitro but also in vivo in orthotopic mouse and zebrafish models, participating to the establishment of a suitable environment for the development of more serious diseases such as cancers. We identified several miRNAs, such as hsa-miR3135b and hsa-miR-21-5p, respectively induced and repressed by DCMU, which alters CD8+ T cell functions. The major advancement of our study is the description of the molecular mechanisms induced by DCMU that control human immune functions.
 
Overall design We used microarray to identify miRNA induced or repressed by DCMU
 
Contributor(s) Delphine F, Pierre A
Citation(s) 35967413
Submission date Nov 23, 2021
Last update date Aug 24, 2022
Contact name Delphine Fradin
E-mail(s) delphine.fradin@inserm.fr
Organization name CRCI2NA, Inserm UMR1307, CNRS UMR6075
Street address 8 quai Moncousu
City Nantes
ZIP/Postal code 44007
Country France
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (18)
GSM5702006 CD8+ T cells exposed to DMSO at 250µM during 24h (rep 1)
GSM5702007 CD8+ T cells exposed to DMSO at 100µM during 24h (rep 1)
GSM5702008 CD8+ T cells exposed to DMSO at 10µM during 24h (rep 1)
Relations
BioProject PRJNA783023

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189443_RAW.tar 12.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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