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| Status |
Public on Nov 30, 2021 |
| Title |
Transcriptomic analyis of isotype specific bone marrow and siLP IgA and IgAKO plasma cells as well as follicular and MZ B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Plasma cell gene expression is driven both by isotype and tissue location. In this series we examine gene expression of bone marrow IgA, IgM and IgG plasma cells as well as IgA plasma cells from small intestine lamina propria. To validate tissue specific gene expression we also include gene expression from lamina propria IgA-/- plasma cells. All plasma cell samples are from Blimp1+/GFP reporter animals and splenic follicular and marginal zone B cell gene expression have been added as reference populations.
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| Overall design |
Samples are double sorted IgM+, IgA+ and IgM/A- BM PCs, IgA+ siLP PCs and PCs from IgAKO siLP as well as splenic follicular and marginal zone B cells. cDNA was prepared from RNA with 3' polyA capture method. Transcript abundance was measured by Kallisto psuedo-alignment and normalized (voom) and differential expression was assessed using limma.
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| Contributor(s) |
Allman D, Wilmore JR, Gaudette BT |
| Citation(s) |
35003110 |
| |
| Submission date |
Nov 23, 2021 |
| Last update date |
Feb 27, 2022 |
| Contact name |
Brian Thomas Gaudette |
| Organization name |
University of Pennsylvania
|
| Department |
Pathology and Lab Medicine
|
| Lab |
Brian Gaudette
|
| Street address |
3620 Hamilton Walk, Pathology and Laboratory Medicine
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA782959 |
| SRA |
SRP347443 |
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