GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE189308 Query DataSets for GSE189308
Status Public on Apr 01, 2022
Title addition of proteasome inhibitor to J774 cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary J774 cells were treated with MG132, epoxomicin and ONX-0912 for 4 hours, and gene expression was evaluated
In order to evaluate the molecular mechanism of inflammatory responses in PRAAS(Proteasome-Associated Autoinflammatory Syndromes ), we established Psmb8-KI mouse that harbors the same mutation in human patients. Psmb8-KI mice showed higher susceptibility to imiquimod-induced skin inflammation (IMS). DNA microarray analysis showed that treatment of J774 cells with proteasome inhibitors increased the expression of the Cxcl9 and Cxcl10 genes, and the skins where imiquimod was painted also expressed both genes at higher levels in Psmb8-KI than control mice. Deficiency in Cxcr3, the gene encoding the receptor of CXCL9 and CXCL10, in wild-type mice did not change IMS susceptibility, while deficiency in Cxcr3 in Psmb8-KI mice ameliorated IMS.
Overall design Blockade of the CXCR3-CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction
Contributor(s) Sasaki Y, Yasutomo K
Citation(s) 35393946
Submission date Nov 22, 2021
Last update date May 10, 2022
Contact name Yuki Sasaki
Phone 81-088-633-7077
Organization name Tokushima University Graduate school of Medicine
Department Department of Immunology and Parasitology
Street address 3-18-15 Kuramoto-cho
City Tokushima
ZIP/Postal code 770-8503
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (4)
GSM5698698 DMSO-1
GSM5698699 MG132-3
GSM5698700 EPOX-4
BioProject PRJNA782498

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189308_RAW.tar 8.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap