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| Status |
Public on Dec 13, 2021 |
| Title |
Emergence and patterning dynamics of mouse definitive endoderm |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The segregation of definitive endoderm (DE) from bi-potent mesendoderm progenitors leads to the formation of two distinct germ layers. Dissecting DE commitment and onset has been challenging as it occurs within a narrow spatio-temporal window in the embryo. Here we employ a novel dual Bra/Sox17 reporter cell line to study DE onset dynamics. We find Sox17 expression initiates in a few isolated cells in vivo within a temporally restricted window. Using 2D and 3D in vitro models, we show that DE cells emerge from mesendoderm progenitors at a temporally regular, but spatially stochastic pattern, which is subsequently arranged by self-sorting of Sox17+ cells. A subpopulation of Bra-high cells commits to a Sox17+ fate independent of external Wnt signal. Self-sorting coincides with up-regulation of E-cadherin but is not necessary for DE differentiation or proliferation. Our in vivo and in vitro results highlight basic rules governing DE onset and patterning through the commonalities and differences between these systems.
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| Overall design |
In this study, we analyzed the dynamics of Sox17 (marker of definitive endoderm) dynamics in vitro and in vivo. We show that majority of these cells originate from T+ population, and that the disperse pattern of Sox17+ cells is a cell-autonomous choice which is free of spatial feedback.
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| Contributor(s) |
Pour M, Kumar AS, Farag N, Bolondi A, Kretzmer H, Walther M, Wittler L, Meissner A, Nachman I |
| Citation(s) |
34988400 |
| |
| Submission date |
Nov 19, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Helene Kretzmer |
| E-mail(s) |
kretzmer@molgen.mpg.de
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| Organization name |
Max Planck Institute for Molecular Genetics
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| Department |
Genome Regulation
|
| Lab |
Meissner Lab
|
| Street address |
Ihnestraße 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA782003 |
| SRA |
SRP346960 |
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