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| Status |
Public on Nov 22, 2021 |
| Title |
Enoxacin shows a broad-spectrum antiviral activity against diverse viruses by enhancing antiviral RNAi in insects |
| Organisms |
Drosophila melanogaster; Drosophila C virus strain EB |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
RNA interference (RNAi) functions as the major host antiviral defense in insects, while less is understood about how to utilize antiviral RNAi in controlling viral infection in insects. Enoxacin belongs to the family of synthetic antibacterial compounds based on a fluoroquinolone skeleton that has been previously found to enhance RNAi in mammalian cells. In this study, we showed that enoxacin efficiently inhibited viral replication of Drosophila C virus (DCV) and Cricket paralysis virus (CrPV) in cultured Drosophila cells. Enoxacin promoted the loading of Dicer-2-processed virus-derived siRNA into the RNA-induced silencing complex, thereby enhancing antiviral RNAi response in infected cells. Moreover, enoxacin treatment elicited an RNAi-dependent in vivo protective efficacy against DCV or CrPV challenge in adult fruit flies. In addition, enoxacin also inhibited replication of flaviviruses, including Dengue virus and Zika virus, in Aedes mosquito cells in an RNAi-dependent manner. Together, our findings demonstrated that enoxacin can enhance RNAi in insects, and enhancing RNAi by enoxacin is an effective antiviral strategy against diverse viruses in insects, which may be exploited as a broad-spectrum antiviral agent to control vector transmission of arboviruses or viral diseases in insect farming.
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| Overall design |
Small RNA profile of virus-infected Drosophila S2 cells.
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| Contributor(s) |
Lyu B, Wang C, Bie Y, Kong J, Wang A, Jin L, Qiu Y, Zhou X |
| Citation(s) |
34908449 |
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| Submission date |
Nov 19, 2021 |
| Last update date |
Feb 21, 2022 |
| Contact name |
Yang Qiu |
| E-mail(s) |
yangqiu@wh.iov.cn
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| Organization name |
Wuhan institue of virology, CAS
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| Street address |
No. 44 Xiaohongshan Central District, Wuchang District,
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430071 |
| Country |
China |
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| Platforms (1) |
| GPL30984 |
Illumina HiSeq 2000 (Drosophila C virus strain EB; Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA781957 |
| SRA |
SRP346934 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189204_RAW.tar |
80.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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