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| Status |
Public on Jul 07, 2022 |
| Title |
Next generation sequencing analysis of mouse adrenal glands after a one-hour dexamethasone treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA sequencing (RNA-seq) was used to identify early transcriptomic responses of the adrenal gland to the 1-hour dexamethasone treatment in mice.
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| Overall design |
Five male mice were IP injected with dexamethason (10 micro gram) and another five mice were IP injected with phosphate-buffered saline. Adrenal glands were collected one hour after the treatment. Adrenal glands were pooled (2 mice and 3 mice) and total RNAs were extracted to make two replicates for each treatment group. Paired-end RNA-seq (PE150) was performed using the Illumina platform.
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| Contributor(s) |
Zheng HS, Daniel JG, Salamat JM, Mackay L, Foradori CD, Kemppainen RJ, Pondugula SR, Tao Y, Huang CJ |
| Citation(s) |
35904237 |
| |
| Submission date |
Nov 19, 2021 |
| Last update date |
Aug 16, 2022 |
| Contact name |
Sophia Zheng |
| E-mail(s) |
sophia.zheng@auburn.edu
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| Organization name |
Auburn University
|
| Department |
Anatomy, Physiology 7 Pharmacology
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| Lab |
Huang Lab
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| Street address |
1130 Wire Road
|
| City |
Auburn |
| State/province |
AL |
| ZIP/Postal code |
36832 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA781856 |
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