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Series GSE189164 Query DataSets for GSE189164
Status Public on Nov 23, 2021
Title Titanium with nanotopography attenuates the osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the application of RNA sequencing to evaluate the effect of titanium (Ti) with nanotopography, compared to Ti control, on the whole transcriptome of osteoblastic cells in isolated cultures grown on these surfaces and to evaluate the effect of Ti with nanotopography, compared to Ti control, on the whole transcriptome of osteoblastic cells, grown on these surfaces, in co-culture with osteoclastic cells.
MC3T3-E1 osteoblastic cells were plated at the density of 1x10^4 cells/well on the Ti discs with nanotopography and Ti control, in 24-well plates in osteogenic medium and kept isolated for 5 days to allow cell adhesion. Osteoclast cells from the RAW 264.7 lineage were cultured on osteoclastogenic medium on ThinCert™ cell culture inserts (Greiner Bio-One), with 0.4 μm pore, at the density of 1x104 cells/membrane, and was also be kept isolated for 5 days to allow cell adhesion. At the end of this period theThinCert™ cell culture inserts (Greiner Bio-One), containing the osteoclast cells were positioned on the osteoblastic cell cultures, thereby establishing indirect co-culture. The controls were osteoblastic cells of the MC3T3-E1 lineage grown on the Ti discs in the absence of osteoclast cells of the RAW 264.7 lineage, i.e., not maintained in co-culture.
Overall design All the experiments were performed considering biological triplicates of each group.
Contributor(s) Bighetti-Trevisan RL, Almeida LO, Castro-Raucci LM, Gordon JA, Tye CE, Stein GS, Lian JB, Stein JL, Rosa AL, Beloti MM
Citation(s) 35012895
Submission date Nov 19, 2021
Last update date Feb 25, 2022
Contact name Rayana Longo Bighetti-Trevisan
Organization name University of São Paulo
Department Basic and Oral Biology
Lab Bone Research Lab
Street address Avenida do Café s/n.
City Ribeirão Preto
State/province São Paulo
ZIP/Postal code 14040903
Country Brazil
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (12)
GSM5695600 MC3T3-E1_Co-culture_Control_R1
GSM5695601 MC3T3-E1_Co-culture_Control_R2
GSM5695602 MC3T3-E1_Co-culture_Control_R3
BioProject PRJNA781837
SRA SRP346891

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Supplementary file Size Download File type/resource
GSE189164_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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