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| Status |
Public on Nov 08, 2023 |
| Title |
Brain glucose induces tolerance of Cryptococcus neoformans to amphotericin B during meningitis |
| Organism |
Cryptococcus neoformans var. grubii H99 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In bacteria, antibiotic tolerance, the ability of a susceptible population to survive high doses of cidal drugs, has been shown to compromise therapeutic outcomes. In comparison, whether fungicide tolerance can be induced by host-derived factors during fungal diseases remains unproven. Here, through a systematic evaluation of metabolite-drug-fungal interactions in the leading fungal meningitis pathogen, Cryptococcus neoformans, we found that glucose, on which the brain depends for fuel, induces fungal tolerance to amphotericin B (AmB) in mouse brain tissue and patient cerebrospinal fluid via the fungal glucose repression activator Mig1. To explore the mechanisms underlying glucose-dependent AmB tolerance mediated by Mig1, we used time-series RNA-seq to evaluate dynamic gene expression in wildtype and mig1Δ fungi after exposure to AmB under both drug-tolerant (glucose) or drug-sensitive (galactose) conditions.
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| Overall design |
24 samples contain wild type, mig1△, three replicates
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/38225460/
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| Contributor(s) |
Chen L, Tian X, Zhang L, Wang W, Hu P, Ma Z, Li Y, Li S, Shen Z, Fan X, Ye L, Ke W, Wu Y, Shui G, Xiao M, He G, Yang Y, Fang W, Bai F, Liao G, Chen M, Lin X, Li C, Wang L |
| Citation(s) |
38225460 |
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| Submission date |
Nov 16, 2021 |
| Last update date |
Feb 07, 2024 |
| Contact name |
lei chen |
| E-mail(s) |
threestonechen@outlook.com
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| Phone |
18066336645
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| Organization name |
the institute of microbiology chinese academy of sciences
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| Street address |
NO.1 Beichen West Road, Chaoyang District, Beijing 100101, China
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| City |
beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL24579 |
Illumina HiSeq 4000 (Cryptococcus neoformans var. grubii H99) |
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| Samples (72)
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| Relations |
| BioProject |
PRJNA780908 |
| SRA |
SRP346394 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE188965_processed_data_Added.xlsx |
3.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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