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Series GSE188853 Query DataSets for GSE188853
Status Public on Jun 03, 2022
Title The function of Wtap in N6-adenosine methylation of mRNAs controls T cell receptor signaling and survival of T cells [m6A-CLIP]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T cell antigen receptor (TCR) signaling controls the development, activation and survival of T cells by involving several layers and numerous mechanisms of gene regulation. N6-methyladenosine (m6A) is the most prevalent mRNA modification affecting splicing, translation and stability of transcripts. Here, we describe the Wtap protein as essential for m6A methyltransferase complex function and reveal its crucial role in TCR signaling. Wtap and m6A methyltransferase functions were required for the differentiation of thymocytes, control of activation-induced death of peripheral T cells and prevention of colitis by enabling gut RORγt+ regulatory T cell function. Transcriptome and epitranscriptomic analyses reveal that m6A modification destabilizes Orai1 and Ripk1 mRNAs. Lack of post-transcriptional repression of the encoded proteins correlated with increased store-operated calcium entry (SOCE) activity and diminished survival of T cells. These findings uncover how m6A modification impacts on TCR signal transduction and determines activation and survival of T cells.
Overall design Examination of transcriptome in WT or Wtap KO CD4+ T cells. m6A-CLIP or YTHDF2-iCLIP experiments were performed on polyA+ RNA from mouse CD4+ cells with 3 or 2 biological replicates, respectively.
Contributor(s) Ito-Kureha T, Heissmeyer V
Citation(s) 35879451
Submission date Nov 15, 2021
Last update date Nov 04, 2022
Contact name Taku Kureha
Phone +49-(0)89-2180-71211
Organization name Ludwig-Maximilians-Universität
Department Institute for Immunology
Lab Molecular Immune Regulation
Street address Grosshaderner Str. 9
City Planegg-Martinsried
ZIP/Postal code 82152
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (5)
GSM5691031 m6A-CLIP1
GSM5691032 m6A-CLIP2
GSM5691033 m6A-CLIP3
This SubSeries is part of SuperSeries:
GSE188854 The function of Wtap in N6-adenosine methylation of mRNAs controls T cell receptor signaling and survival of T cells
BioProject PRJNA780524
SRA SRP346159

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Supplementary file Size Download File type/resource 3.8 Mb (ftp)(http) BW 3.8 Mb (ftp)(http) BW 45.2 Mb (ftp)(http) BW 46.4 Mb (ftp)(http) BW
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