|
| Status |
Public on May 03, 2023 |
| Title |
Establish a rapid and efficient chemical reprogramming platform (ATAC-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chemically reprogramming somatic cells to iPSCs is time-consuming and low-efficiency. Here, we discribe a rapid chemical reprogramming condition enabling generating iPSCs from MEFs in 12 days. To further investigate the mechemisms and draw an epigenetic maps during the rapid chemical reprogramming, we performed time-course RNA-seq, ATAC-seq, RRBS and CUT&Tag (H3K4me3, H3K18la, H3K9me3, H3K27me3 and H3K27ac).
|
| |
|
| Overall design |
Cells were collected every 2 days of reprogramming along with riPSCs were used for ATAC-seq.
|
| Web link |
https://www.nature.com/articles/s41556-023-01193-x
|
| |
|
| Contributor(s) |
Chen X, Lu Y, Zhu S |
| Citation(s) |
37550515 |
| |
| Submission date |
Nov 15, 2021 |
| Last update date |
Aug 09, 2023 |
| Contact name |
Yunkun Lu |
| E-mail(s) |
kevenloo1@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Department |
Life Sciences Institute
|
| Street address |
866 Yuhangtang Rd.
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE188834 |
Establish a rapid and efficient chemical reprogramming platform |
|
| Relations |
| BioProject |
PRJNA780479 |
| SRA |
SRP346131 |
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