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Series GSE18863 Query DataSets for GSE18863
Status Public on Nov 04, 2009
Title KIAA1718 is a dual specific histone demethylase involved in early neural development
Organism Mus musculus
Experiment type Expression profiling by array
Summary Histone methylation is a complex posttranslational modification regulating transcription and chromatin dynamics, and plays important roles in development and disease processes. Recent studies indicate that histone lysine methylation is a reversible modification and several JmjC-domain-containing proteins have been identified as histone lysine demethylases. Here we show that KIAA1718, a JmjC-domain-containing protein, is a histone demethylase. KIAA1718 demethylated dimethylation of H3 lysine 9 and 27 (H3K9me2 and H3K27me2), two important epigenetic marks associated with embryonic development6-10. In mouse embryonic stem cells (ESCs), KIAA1718 expression increased at the early phase of neural differentiation. RNA interference inhibition of KIAA1718 blocked neural differentiation and the effect was rescued by wild-type human gene, but not by a catalytically inactive mutant. In chick embryos, KIAA1718 is exclusively expressed in the epiblast cells of the primitive streak, an organizer contributing to neural induction. Knockdown of KIAA1718 resulted in defects in neural formation, while its overexpression led to neural expansion. The pro-neural effect is mediated through transcriptional activation of FGF4, a growth factor implicated in neural induction. Thus, our study identifies a dual specific histone demethylase as a novel epigenetic factor integrated with growth factor signaling, which has important roles in the early neural development.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series.
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Submission date Nov 03, 2009
Last update date Feb 11, 2019
Contact name Huang Chengyang
Organization name Institute of biochemistry and cell biology
Street address Yue Yang Road 320
City shanghai
ZIP/Postal code 200032
Country China
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (8)
GSM465863 RNAi Luc-1
GSM465864 D0 embryonic stem (ES) cells
GSM465865 D6 embryoid body (EB) cells
This SuperSeries is composed of the following SubSeries:
GSE18771 Mouse KIAA1718 knockdown ES cells
GSE18772 Mouse ES cell neural differentiation
BioProject PRJNA121113

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Supplementary file Size Download File type/resource
GSE18863_RAW.tar 38.9 Mb (http)(custom) TAR (of CEL, CHP, TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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