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| Status |
Public on Dec 15, 2021 |
| Title |
Retinoic acid signaling mediates peripheral cone photoreceptor survival in a mouse model of retina degeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cone photoreceptors in retina degeneration mouse models degenerate from the center to the periphery. However, the far peripheral cones survive long-term. We found that retinoic acid signaling is both necessary and sufficient for cone survival.
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| Overall design |
Bulk RNA-seq of CAR+ cones and Muller glia was performed on P30 central and peripheral retinas. Briefly, the central one-third and peripheral one-third retinal tissues were dissected out from 3 mice. The tissue was dissociated into single cells and labeled with anti-CAR antibody. The CAR+ central and peripheral cells were FACS-isolated, and the RNA was extracted.
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| Contributor(s) |
Amamoto R, Cepko CL |
| Citation missing |
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| Submission date |
Oct 26, 2021 |
| Last update date |
Dec 15, 2021 |
| Contact name |
Ryoji Amamoto |
| E-mail(s) |
ryoji_amamoto@hms.harvard.edu
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| Phone |
6177498955
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| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Cepko
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA774789 |
| SRA |
SRP343252 |
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