|
| Status |
Public on Oct 25, 2021 |
| Title |
Decoding Expression Dynamics of Protein and Transcriptome at the Single Cell Level through Multi-Omics Sequencing in Paired Picoliter Chambers |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Simultaneous analysis of mRNAs and proteins at the single-cell level provides information about the dynamics and correlations of gene and protein expressions in individual cells, enabling comprehensive study of cellular heterogeneity and expression patterns. Here, we present a platform for high-throughput cellular indexing of mRNAs and proteins, named multi-Paired-seq, with high cell utilization, accurate molecular measurement and low cost. Based on hydrodynamic differential flow resistance, multi-Paired-seq largely improves cell utilization (>95%). Combined with pump/valve structure, cell-free antibodies and mRNAs can be removed completely for highly accurate detection. The picoliter reaction chambers allow higher detection sensitivity and lower sequencing cost. By using multi-Paired-seq, more elaborate classifications of breast cancers are identified according to multimodal measurements, and the expression correlations between mRNAs and proteins under altered conditions are quantified. Multi-Paired-seq provides multi-modal measurements at the single cell level, which offers a new tool for cell biology, developmental biology, drug discovery and precision medicine.
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| |
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| Overall design |
mRNA profiles of different cancer cell lines.
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| |
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| Contributor(s) |
Xu X, Zhang M, Zhang X, Yang C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 22, 2021 |
| Last update date |
Oct 26, 2021 |
| Contact name |
Xing Xu |
| E-mail(s) |
xuxing940329@126.com
|
| Phone |
086-18850013503
|
| Organization name |
Xiamen University
|
| Street address |
422 Road, Siming District, Xiamen, China
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361005 |
| Country |
China |
| |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (19)
|
| GSM5651031 |
4T1-MCF7 mixed multi-omics sample RNA |
| GSM5651032 |
4T1-MCF7 mixed multi-omics sample protein |
| GSM5651033 |
4T1-MCF7 mixed single-omics sample RNA |
| GSM5651034 |
MCF7 multi-omics sample RNA _rep1 |
| GSM5651035 |
MCF7 multi-omics sample protein _rep1 |
| GSM5651036 |
MCF7 multi-omics sample RNA _rep2 |
| GSM5651037 |
MCF7 multi-omics sample protein _rep2 |
| GSM5651038 |
SK-BR-3 multi-omics sample RNA _rep1 |
| GSM5651039 |
SK-BR-3 multi-omics sample protein _rep1 |
| GSM5651040 |
SK-BR-3 multi-omics sample RNA _rep2 |
| GSM5651041 |
SK-BR-3 multi-omics sample protein _rep2 |
| GSM5651042 |
MDA-MB-231 multi-omics sample RNA _rep1 |
| GSM5651043 |
MDA-MB-231 multi-omics sample protein _rep1 |
| GSM5651044 |
MDA-MB-231 multi-omics sample RNA _rep2 |
| GSM5651045 |
MDA-MB-231 multi-omics sample protein _rep2 |
| GSM5651046 |
Drug-treated SK-BR-3 multi-omics sample RNA _rep1 |
| GSM5651047 |
Drug-treated SK-BR-3 multi-omics sample protein _rep1 |
| GSM5651048 |
Drug-treated SK-BR-3 multi-omics sample RNA _rep2 |
| GSM5651049 |
Drug-treated SK-BR-3 multi-omics sample protein _rep2 |
|
| Relations |
| BioProject |
PRJNA773743 |
| SRA |
SRP342705 |
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