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Series GSE186297 Query DataSets for GSE186297
Status Public on Jan 19, 2022
Title Effects of DNA topoisomerase inhibitor acriflavine on endothelial gene expression under hypoxic conditions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-Seq of human endothelial cells treated with acriflavine (ACF) with or without hypoxia revealed massive changes on gene expression. These were non-randomly and potentially due to DNA topoisomerase inhibition rather than HIF inhibition. Surprisingly, in contrast to protein-coding genes, RNA-Seq yielded that an exceeding number of lncRNAs is upregulated.
Overall design Examination of acriflavine treatment in HUVECs under hypoxia (16h, 1% O2)
Contributor(s) Seredinski S, Günther S, Brandes RP, Leisegang MS
Citation(s) 35228897, 36700983
Submission date Oct 21, 2021
Last update date Feb 07, 2023
Contact name Stefan Günther
Organization name MPI for heart and lung research
Street address ludwigtr. 43
City bad nauheim
ZIP/Postal code 61231
Country Germany
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (6)
GSM5643363 Hypoxia_DMSO_HUVEC_1 [Hyp-DMSO_1]
GSM5643364 Hypoxia_DMSO_HUVEC_2 [Hyp-DMSO_2]
GSM5643365 Hypoxia_DMSO_HUVEC_3 [Hyp-DMSO_3]
BioProject PRJNA773246
SRA SRP342506

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Supplementary file Size Download File type/resource
GSE186297_counts.matrix.norm_anno.txt.gz 13.9 Mb (ftp)(http) TXT
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