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Series GSE186064 Query DataSets for GSE186064
Status Public on Oct 01, 2023
Title Dgcr8 function in the anterior second heart field
Platform organisms Mus musculus; synthetic construct
Sample organism Mus musculus
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Morphogenesis of the heart is a complex process that relies on the precise gene expression and gene expression regulators during embryonic development. Dgcr8 is a gene involved in cardiac morphogenesis and it is in the chromosomal region deleted in 22q11.2DS patients. In order to study Dgcr8 function on heart development, we inactivated this gene in the cardiac progenitor cells of mouse embryos and did expression profiling of the long RNAs and miRNAs.
We used microarrays to detail the global programme of gene expression downstream of DGCR8 underlying cardiac development.
Overall design Mouse embryos were used at embryonic day E9.5 to microdisect two different cardiac tissues, the cardiac progenitor cells located at the back of the heart tube (aSHF) and the outflow tract and right ventricle (OFT-RV) from the heart tube itself. Several pools of each tissue from wild type or mutants embryos were processed for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain pools combining several embryos of the same genotype per replicate in order to obtain a more accurate representation of the expression profile in the embryo population. To that end, we have pooled embryos of the same genotype from at least five different litters. Thirty different pools were obtained, one per array described in the sample list.
Contributor(s) Racedo S, Morrow B
Citation(s) 38110169
Submission date Oct 18, 2021
Last update date Jan 02, 2024
Contact name Silvia Elisa Racedo
Organization name Albert Einstein College of Medicine
Department Genetics
Lab Morrow Lab
Street address 1301 Morris Park Ave
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
Platforms (2)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (30)
GSM5630037 aSHF, wild-type, biological rep1 (mRNA)
GSM5630038 aSHF, wild-type, biological rep2 (mRNA)
GSM5630039 aSHF, wild-type, biological rep3 (mRNA)
BioProject PRJNA772262

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186064_Gene_expression_OFT-RV.tsv.gz 1.2 Mb (ftp)(http) TSV
GSE186064_Gene_expression_aSHF.tsv.gz 1.2 Mb (ftp)(http) TSV
GSE186064_Matrix_Gene_expression_OFT-RV.txt.gz 1012.1 Kb (ftp)(http) TXT
GSE186064_Matrix_Gene_expression_aSHF.txt.gz 1008.3 Kb (ftp)(http) TXT
GSE186064_Matrix_miRNA_expression_OFT-RV.txt.gz 103.3 Kb (ftp)(http) TXT
GSE186064_Matrix_miRNA_expression_aSHF.txt.gz 133.5 Kb (ftp)(http) TXT
GSE186064_RAW.tar 78.4 Mb (http)(custom) TAR (of CEL)
GSE186064_miRNA_expression_OFT-RV.tsv.gz 204.6 Kb (ftp)(http) TSV
GSE186064_miRNA_expression_aSHF.tsv.gz 201.8 Kb (ftp)(http) TSV
Processed data included within Sample table
Processed data are available on Series record

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