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Series GSE186004 Query DataSets for GSE186004
Status Public on Dec 18, 2021
Title Heat Shock Factor 1 (HSF1) regulates ESR1 action in breast cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Heat shock factor 1 (HSF1) is a key regulator of transcriptional responses to proteotoxic stress. It has been recently linked to signaling of estrogen via ESR1. To study the cooperation of HSF1 and ESR1 in the transcriptional response to estrogen, we established estrogen receptor (ER)-positive breast cancer cell lines with reduced HSF1 levels using specific shRNA or CRISPR/Cas9 approach. HSF1 deficiency led to the inhibition of the mitogenic effect of estrogen in MCF7 and T47D cells. RNA-seq analyses revealed that the stimulatory effect of E2 on the transcriptome was smaller in HSF1-deficient MCF7 cells. This could partially result from the higher basal expression of E2-dependent genes in these cells as a consequence of the enhanced binding of unliganded ESR1 to chromatin, which was revealed by ChIP-seq analyses. Thus, we postulate that some fraction of ESR1 could be released from the inhibitory complex with HSP90 and gain transcriptional competence without E2-stimulation.
Overall design MCF7 cells with different status of HSF1 (HSF1+/+ and HSF1-/-) were untreated or treated with 10 nM E2 for 4 hour. Then total RNA was isolated from each sample using the Direct-ZolTM RNA MiniPrep Kit (Zymo Research), digested with DNase I (Worthington Biochemical Corporation) and next-generation sequenced using the Illumina protocol. For each experimental point, three independent biological repeats were done including RNA isolation and sequencing.
Contributor(s) Vydra N, Janus P, Widlak W, Stokowy T
Citation(s) 34783649
Submission date Oct 15, 2021
Last update date Jan 01, 2022
Contact name Tomasz Stokowy
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (12)
GSM5628758 MCF7 cells, HSF1+/+_control repeat 1
GSM5628759 MCF7 cells, HSF1+/+_control repeat 2
GSM5628760 MCF7 cells, HSF1+/+_control repeat 3
BioProject PRJNA771684
SRA SRP341615

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Supplementary file Size Download File type/resource
GSE186004_Counts_genocode_v26_WWidlak_211014.txt.gz 5.4 Mb (ftp)(http) TXT
GSE186004_Normalized_RNA_211014.txt.gz 2.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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