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| Status |
Public on Aug 11, 2023 |
| Title |
Genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in CTRLcas9 and SCARB2cas9 group. Inspection of multiple individual traces and global analyses showed that SCARB2cas9 globally reduced association of c-Myc with core promoters of its target genes.
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| Overall design |
we precipitated chromatin with tag antibody to CTRLcas9 and SCARB2cas9 HepG2 cells, and sequenced the precipitated material. SCARB2cas9 reduced association of c-Myc with core promoters of its target genes
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| Contributor(s) |
Li K, Jiang J, Wang F, Gao Y, Xue S |
| Citation(s) |
37739936 |
| |
| Submission date |
Oct 13, 2021 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Yang Gao |
| E-mail(s) |
yxgyang@126.com
|
| Phone |
18238837867
|
| Organization name |
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
Dongcheng District, Tiantan Xili
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE185844 |
Scavenger Receptor SCARB2 Drives Hepatic Carcinoma Initiation by Enhancing MYC Transcriptional Activity and Supporting Cancer Stem Cell Traits. |
|
| Relations |
| BioProject |
PRJNA770980 |
| SRA |
SRP341212 |
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