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Series GSE18582 Query DataSets for GSE18582
Status Public on Feb 16, 2010
Title Nuclear retention of fission yeast dicer is a prerequisite for RNAi-mediated heterochromatin assembly
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary RNAseIII ribonucleases act at the heart of RNA silencing pathways by processing precursor RNAs into mature microRNAs and siRNAs. In the fission yeast Schizosaccharomyces pombe, siRNAs are generated by the RNAseIII enzyme Dcr1 and are required for heterochromatin formation. In this study, we have analyzed the subcellular localization of Dcr1 and found that it accumulates in the nucleus and is enriched at the nuclear periphery. Nuclear accumulation of Dcr1 depends on a short motif which constrains nuclear export promoted by the double-stranded RNA binding domain of Dcr1. Absence of this motif renders Dcr1 mainly cytoplasmic and is accompanied by remarkable changes in gene expression and failure to assemble heterochromatin. Our findings suggest that dicer proteins are shuttling proteins and that the steady-state subcellular levels can be shifted towards either compartment. This has implications for the mechanism of RNAi-mediated heterochromatin assembly and the spatial organization of RNA silencing pathways in general.
 
Overall design Small RNA libraries from total RNA isolations of wild-type, dcr1Delta and dcr1DeltaC33 cells and subjected to high-throughput sequencing.
 
Contributor(s) Emmerth S, Schober H, Gaidatzis D, Jacobeit K, Bühler M
Citation(s) 20152181
Submission date Oct 15, 2009
Last update date May 15, 2019
Contact name Dimos Gaidatzis
E-mail(s) d.gaidatzis@fmi.ch
Organization name Friedrich Miescher Institute
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL9453 Illumina Genome Analyzer II (Schizosaccharomyces pombe)
Samples (3)
GSM462212 Wild-type
GSM462213 dcr1Delta
GSM462214 dcr1DeltaC33
Relations
SRA SRP001891
BioProject PRJNA120293

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Supplementary file Size Download File type/resource
GSE18582_RAW.tar 3.5 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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