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| Status |
Public on Nov 17, 2009 |
| Title |
sRNAs in Rhizobium etli differentially expressed during free-living and host-associated growth |
| Organism |
Rhizobium etli CFN 42 |
| Experiment type |
Non-coding RNA profiling by genome tiling array
Expression profiling by genome tiling array
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| Summary |
89 small non-coding RNAs (ncRNAs) were identified in the soil-dwelling alpha-proteobacterium Rhizobium etli by comparing an extensive compilation of ncRNA predictions to intergenic expression data of a whole-genome tiling array. The differential expression levels of some of these ncRNAs during free-living growth and during interaction with the eukaryotic host plant may indicate a role in adaptation to changing environmental conditions.
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| Overall design |
In order to study expression in the free-living state, wild-type R. etli CFN42 was grown at 30˚C in acid minimal salts medium supplied with 10 mM NH4Cl and 10 mM succinate while monitoring the optical density (OD) of the culture. Samples were taken at OD600 = 0.3, 0.7 and 6 hours after reaching the maximum OD, representing early/late exponential and stationary phase, respectively. In order to study gene expression during host-associated growth, common bean plants (Phaseolus vulgaris cv. Limburgse vroege) were cultivated and inoculated as described previously. Nodules were harvested 2 and 3 weeks after inoculation and the bacteroids were purified by differential centrifugation.
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| Contributor(s) |
Vercruysse M, Fauvart M, Michiels J, Cloots L, Engelen K |
| Citation(s) |
20089193 |
| |
| Submission date |
Oct 15, 2009 |
| Last update date |
Dec 19, 2013 |
| Contact name |
Kristof Engelen |
| Organization name |
KULeuven
|
| Lab |
CMPG
|
| Street address |
Kasteelpark Arenberg 20
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| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL9409 |
Rhizobium etli CFN 42 385K 071203_ReCFN42JM_Tiling |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA120289 |
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