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Series GSE185750 Query DataSets for GSE185750
Status Public on Oct 19, 2021
Title Pancreatic Tumor Microenvironment Maintains Tumor Growth through Nucleosides Secretion
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cancer-associated fibroblasts (CAFs) are one of the most prominent and active components in the pancreatic tumor microenvironment. Our data show that CAFs are critical for PDAC survival upon glutamine deprivation. Specifically, we uncovered a role for nucleosides, which are secreted by CAFs through autophagy in an NUFIP1-depenent manner, increased glucose utilization and promoted growth of PDAC. Moreover, we demonstrate that CAF-derived nucleosides induced glucose consumption under glutamine-deprived conditions and displayed a dependence on MYC. Using an orthotopic mouse model of PDAC, we found that inhibiting nucleoside secretion by targeting NUFIP1 in the stroma reduced tumor weight. This finding highlights a previously unappreciated metabolic network within pancreatic tumors in which diverse nutrients are used to promote growth in an austere tumor microenvironment.
 
Overall design 2 samples.14837 PDAC cells were treated with CAF conditional medium or 14837 conditional medium. Cells' RNA was harvested using Trizol reagent. 2 ug of total RNA is used for the construction of sequencing libraries by Novaseq 6000.
 
Contributor(s) Yuan M, Zhao Y
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Submission date Oct 12, 2021
Last update date Oct 19, 2021
Contact name YU ZHANG
E-mail(s) 1410305236@pku.edu.cn
Phone 01082805861
Organization name Peking Univ.
Street address XUEYUANLU No.38 Haidian District, PKUHSC Anatomy Building
City 北京
State/province non-US Resident
ZIP/Postal code 100191
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5623195 14837 CM
GSM5623196 mCAF CM
Relations
BioProject PRJNA770663
SRA SRP341042

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Supplementary file Size Download File type/resource
GSE185750_Tumor2VS3.txt.gz 131.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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