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Series GSE185570 Query DataSets for GSE185570
Status Public on May 16, 2022
Title Expression data from Saccharomyces cerevisiae to evaluate the impact of suppressors of Aβ42 cytotoxicity on recombinant protein production
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary High-level production of pharmaceutical proteins in industrial microorganism is often limited due to the increased cellular stress from misfolded proteins or protein aggregates. Here, we explore the feasibility of applying a yeast Alzheimer’s disease (AD) model with accumulation of amyloid-β peptides (Aβ42), which presents similar phenotypes of cellular stress. We utilize the suppressors of Aβ42 cytotoxicity as potential metabolic engineering targets to improve industrial protein production. The transcriptomics analyses provide new insights towards developing synthetic yeast cell factories for biosynthesis of valuable pharmaceutical proteins.
 
Overall design The goal of the present study is to evaluate the impact of suppressors of Aβ42 cytotoxicity on recombinant protein production in yeast. Four engineered strains (A02, A05, A08, and A16) and control strain A01 were collected from early exponential phase (OD600nm 1.0). Samples were taken from three biological replicate cultures.
 
Contributor(s) Chen X, Li X, Ji B, Wang Y, Ishchuk OP, Vorontsov E, Engqvist MK, Siewers V, Petranovic D
Citation(s) 35508267, 35677454
Submission date Oct 08, 2021
Last update date Jun 24, 2022
Contact name Xin Chen
E-mail(s) cxin@chalmers.se
Organization name Chalmers University of Technology
Department Department of Biology and Biological Engineering
Street address Kemigården 4
City Göteborg
ZIP/Postal code 41296
Country Sweden
 
Platforms (1)
GPL21656 Illumina HiSeq 4000 (Saccharomyces cerevisiae)
Samples (15)
GSM5618482 A01_RNASeq, biological rep1
GSM5618483 A01_RNASeq, biological rep2
GSM5618484 A01_RNASeq, biological rep3
Relations
BioProject PRJNA769694
SRA SRP340553

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Supplementary file Size Download File type/resource
GSE185570_Processed_data_Xin.txt.gz 253.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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