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Status |
Public on May 16, 2022 |
Title |
Expression data from Saccharomyces cerevisiae to evaluate the impact of suppressors of Aβ42 cytotoxicity on recombinant protein production |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
High-level production of pharmaceutical proteins in industrial microorganism is often limited due to the increased cellular stress from misfolded proteins or protein aggregates. Here, we explore the feasibility of applying a yeast Alzheimer’s disease (AD) model with accumulation of amyloid-β peptides (Aβ42), which presents similar phenotypes of cellular stress. We utilize the suppressors of Aβ42 cytotoxicity as potential metabolic engineering targets to improve industrial protein production. The transcriptomics analyses provide new insights towards developing synthetic yeast cell factories for biosynthesis of valuable pharmaceutical proteins.
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Overall design |
The goal of the present study is to evaluate the impact of suppressors of Aβ42 cytotoxicity on recombinant protein production in yeast. Four engineered strains (A02, A05, A08, and A16) and control strain A01 were collected from early exponential phase (OD600nm 1.0). Samples were taken from three biological replicate cultures.
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Contributor(s) |
Chen X, Li X, Ji B, Wang Y, Ishchuk OP, Vorontsov E, Engqvist MK, Siewers V, Petranovic D |
Citation(s) |
35508267, 35677454 |
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Submission date |
Oct 08, 2021 |
Last update date |
Jun 24, 2022 |
Contact name |
Xin Chen |
E-mail(s) |
cxin@chalmers.se
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Organization name |
Chalmers University of Technology
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Department |
Department of Biology and Biological Engineering
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Street address |
Kemigården 4
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City |
Göteborg |
ZIP/Postal code |
41296 |
Country |
Sweden |
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Platforms (1) |
GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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Samples (15)
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GSM5618485 |
A02_RNASeq, biological rep1 |
GSM5618486 |
A02_RNASeq, biological rep2 |
GSM5618487 |
A02_RNASeq, biological rep3 |
GSM5618488 |
A05_RNASeq, biological rep1 |
GSM5618489 |
A05_RNASeq, biological rep2 |
GSM5618490 |
A05_RNASeq, biological rep3 |
GSM5618491 |
A08_RNASeq, biological rep1 |
GSM5618492 |
A08_RNASeq, biological rep2 |
GSM5618493 |
A08_RNASeq, biological rep3 |
GSM5618494 |
A16_RNASeq, biological rep1 |
GSM5618495 |
A16_RNASeq, biological rep2 |
GSM5618496 |
A16_RNASeq, biological rep3 |
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Relations |
BioProject |
PRJNA769694 |
SRA |
SRP340553 |
Supplementary file |
Size |
Download |
File type/resource |
GSE185570_Processed_data_Xin.txt.gz |
253.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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