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| Status |
Public on Oct 08, 2021 |
| Title |
Gene expression in human corneal epithelial cells (hCECs) with different post-mortem intervals and cultivated in monoculture or used for human tissue‐engineered corneas (hTECs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we evaluated the effects of the post-mortem interval, defined as the number of days elapsed between the death of a donor and the isolation of hCECs, on the properties of hCECs.
Using gene profiling, we compared the mRNA profiles of passage 1 hCECs (10 populations) cultivated in moculture with different post-mortem intervals and passage 2 hCECs (6 populations) with different post-mortem intervals from hTECs.
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| Overall design |
Ten hCECs populations (passage 1) with different post-mortem intervals cultivated in monoculutre and six hCECs populations (passage 2) with different post-mortem intervals from hTECs are analyzed by gene profiling
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| Contributor(s) |
Le-Bel G, Desjardins P, Gross C, Cortez Ghio S, Couture C, Germain L, Guérin S |
| Citation(s) |
36078126 |
| |
| Submission date |
Oct 06, 2021 |
| Last update date |
Oct 07, 2022 |
| Contact name |
Gaëtan Le-Bel |
| E-mail(s) |
gaetan.lebel17@gmail.com
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| Organization name |
CUO-Recherche
|
| Lab |
Sylvain Guérin
|
| Street address |
Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
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| City |
Québec |
| State/province |
Quebec |
| ZIP/Postal code |
G1S 4L8 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (16)
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| GSM5615658 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_0day_001_rep1 |
| GSM5615659 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_1day_002_rep1 |
| GSM5615660 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_2days_003_rep1 |
| GSM5615661 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_3days_004_rep1 |
| GSM5615662 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_4days_005_rep1 |
| GSM5615663 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_14days_006_rep1 |
| GSM5615664 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_15days_007_rep1 |
| GSM5615665 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_17days_008_rep1 |
| GSM5615666 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_18days_009_rep1 |
| GSM5615667 |
EyeBank_cultured_hCECs_monoculture_interval_post-mortem_19days_010_rep1 |
| GSM5615668 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_0day_001_rep1 |
| GSM5615669 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_1day_002_rep1 |
| GSM5615670 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_2days_003_rep1 |
| GSM5615671 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_17days_008_rep1 |
| GSM5615672 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_18days_009_rep1 |
| GSM5615673 |
EyeBank_cultured_hCECs_from_hTECs_interval_post-mortem_19days_010_rep1 |
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| Relations |
| BioProject |
PRJNA769127 |
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