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Series GSE185399 Query DataSets for GSE185399
Status Public on Mar 05, 2022
Title Effect of the primed exposure of Diesel CAST model aerosol in air-liquid interface BEAS-2B cells exposed to native birch pollen
Organism Homo sapiens
Experiment type Expression profiling by array
Summary BEAS-2B cells, at air-liquid interface, were exposed to Diesel CAST model aerosol in an vitro exposure system and, later, to native birch pollen using a Pollen Sedimentation Chamber stabilished before. The same exposure was performed, without the primed anthropogenic exposure. The goal was to understand the effect of pre-exposure to a model diesel aerosol in allergic sensitization, using a model stabilished that mimics a real life exposure as closer as possible.
Overall design For the combined exposure, BEAS-2B, at air-liquid interface, were exposed to Diesel CAST model aerosol for 2 hours and after 24 hours exposed to 4 mg whole birch pollen for different incubation times (2h25 min, 6h50 min and 24h). The cells were seeded in 24mm Transwells 48 hours before exposure and kept in air lift conditions 24h before start. Effect of combined expousre were referenced against cells exposed to clean air and pollen. For the pollen exposure alone, BEAS-2B, also at air-liquid interface, were exposed to 4 mg birch pollen for the indicated times. Here, effects of birch whole pollen were referenced against cells exposed to air. Three replicates were performed for each incubation time.
Contributor(s) Candeias J, Zimmermann EJ, Bisig C, Gawlitta N, Oeder S, Groeger T, Zimmermann R, Schmidt-Weber CB, Buters J
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Submission date Oct 05, 2021
Last update date Mar 05, 2022
Contact name Joana Candeias
Organization name Technical University Munich & Helmholtz Center Munich
Department Center Allergy and Environment (ZAUM)
Lab Prof. Dr. Jeroen Buters Lab
Street address Biedersteinerstr. 29
City Munich
ZIP/Postal code 80802
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (40)
GSM5614065 BEAS_4mg_2h25min_Air_rep1
GSM5614066 BEAS_4mg_2h25min_Air_rep2
GSM5614067 BEAS_4mg_2h25min_Air_rep3
BioProject PRJNA768879

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185399_RAW.tar 490.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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