BEAS-2B cells, at air-liquid interface, were exposed to Diesel CAST model aerosol in an vitro exposure system and, later, to native birch pollen using a Pollen Sedimentation Chamber stabilished before. The same exposure was performed, without the primed anthropogenic exposure. The goal was to understand the effect of pre-exposure to a model diesel aerosol in allergic sensitization, using a model stabilished that mimics a real life exposure as closer as possible.
For the combined exposure, BEAS-2B, at air-liquid interface, were exposed to Diesel CAST model aerosol for 2 hours and after 24 hours exposed to 4 mg whole birch pollen for different incubation times (2h25 min, 6h50 min and 24h). The cells were seeded in 24mm Transwells 48 hours before exposure and kept in air lift conditions 24h before start. Effect of combined expousre were referenced against cells exposed to clean air and pollen. For the pollen exposure alone, BEAS-2B, also at air-liquid interface, were exposed to 4 mg birch pollen for the indicated times. Here, effects of birch whole pollen were referenced against cells exposed to air. Three replicates were performed for each incubation time.