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| Status |
Public on Oct 01, 2022 |
| Title |
RNA sequencing of slow-and fast-cycling epidermal stem cells vs hair follicle stem cells in young vs old mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study was performed to compare transcriptomic changes in the heterogeneous mouse skin epidermal stem cells and hair follicle stem cells (HFSC) populations during chronological aging. Slow-cycling stem cells (label retaining cells, LRCs), fast-cycling stem cells (non-label retaining cells, nLRCs) and hair follicle stem cells express unique gene signatures in young age (2 months old) and have independent stem cell identities. The changes in aging stem cells lineage identities have been a topic of discussion and here we examined if distinct stem cells cycling speed affects their aging process by comparing the transcriptomes of slow-and fast-cycling epidermal stem cells. Our data indicates the loss of unique stem cell identities in aging slow or fast-cycling epidermal stem cells or HFSC at 2 years of age with intermediary effects seen at 1.5 year old.
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| Overall design |
mRNA expression analysis from slow-and fast-cycling epidermal stem cells and hair follicle stem cells at 2 months old, 1.5 year old and 2 years old.
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| Contributor(s) |
Changarathil G, Raja E, Sada A, Yanagisawa H |
| Citation(s) |
36278510 |
| |
| Submission date |
Sep 30, 2021 |
| Last update date |
Jan 05, 2023 |
| Contact name |
Erna Raja |
| Organization name |
University of Tsukuba
|
| Department |
TARA center
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| Lab |
Yanagisawa
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA767627 |
| SRA |
SRP339564 |
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