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Status |
Public on Oct 01, 2022 |
Title |
RNA sequencing of epidermal stem cells in Fbln7 WT vs Fbln7 KO mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
This study was performed to compare the transcriptome of epidermal stem cells in young (2 months old) and middle aged (1 year old) fibulin 7 wild type (WT) compared to fibulin 7 knockout (KO) mice. Fbln7 gene was an ECM gene significantly upregulated in aging fast-cycling stem cells and this RNA-sequencing was done to investigate the role of Fbln7 in aging epidermal stem cells. Data indicates differences in gene expressions occuring between WT and KO at 1 year old but not at 2 months old, suggesting an age dependent role of fibulin 7. At 1 year old, other matrix-related and remodelling genes were affected, together with inflammatory responses of stem cells and lineage fate abberations, as in the case of 2 years old fast-cycling epidermal stem cells.
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Overall design |
mRNA expression analysis from epidermal stem cells of Fbln7 WT or KO mice at 2 months old compared to 1 year old
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Contributor(s) |
Changarathil G, Oinam L, Raja E, Sada A, Yanagisawa H |
Citation(s) |
36278510 |
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Submission date |
Sep 30, 2021 |
Last update date |
Jan 05, 2023 |
Contact name |
Erna Raja |
Organization name |
University of Tsukuba
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Department |
TARA center
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Lab |
Yanagisawa
|
Street address |
1-1-1 Tennodai
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City |
Tsukuba |
ZIP/Postal code |
305-8577 |
Country |
Japan |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (13)
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Relations |
BioProject |
PRJNA767626 |
SRA |
SRP339562 |