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Series GSE185086 Query DataSets for GSE185086
Status Public on Oct 01, 2022
Title RNA sequencing of epidermal stem cells in Fbln7 WT vs Fbln7 KO mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study was performed to compare the transcriptome of epidermal stem cells in young (2 months old) and middle aged (1 year old) fibulin 7 wild type (WT) compared to fibulin 7 knockout (KO) mice. Fbln7 gene was an ECM gene significantly upregulated in aging fast-cycling stem cells and this RNA-sequencing was done to investigate the role of Fbln7 in aging epidermal stem cells. Data indicates differences in gene expressions occuring between WT and KO at 1 year old but not at 2 months old, suggesting an age dependent role of fibulin 7. At 1 year old, other matrix-related and remodelling genes were affected, together with inflammatory responses of stem cells and lineage fate abberations, as in the case of 2 years old fast-cycling epidermal stem cells.
 
Overall design mRNA expression analysis from epidermal stem cells of Fbln7 WT or KO mice at 2 months old compared to 1 year old
 
Contributor(s) Changarathil G, Oinam L, Raja E, Sada A, Yanagisawa H
Citation(s) 36278510
Submission date Sep 30, 2021
Last update date Jan 05, 2023
Contact name Erna Raja
Organization name University of Tsukuba
Department TARA center
Lab Yanagisawa
Street address 1-1-1 Tennodai
City Tsukuba
ZIP/Postal code 305-8577
Country Japan
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (13)
GSM5605975 2mths_WT_1
GSM5605976 2mths_WT_2
GSM5605977 2mths_WT_3
Relations
BioProject PRJNA767626
SRA SRP339562

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185086_Fbln7_WT_KO_supplementary.xlsx 20.9 Mb (ftp)(http) XLSX
GSE185086_RAW.tar 6.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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