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Series GSE184711 Query DataSets for GSE184711
Status Public on Sep 26, 2021
Title γH2AX-associated RNA in mouse spermatocytes
Organism Mus musculus
Experiment type Other
Summary In the testis of the mice, most spermatogonia are in pachytene stage. We have harvested spermatogonia from 3-week-old mice. As γH2AX is the marker of the XY body, we performed RNA-seq following RNA-ChIP using anti-γH2AX antibody in germ cells to report the γH2AX associated RNA in mouse spermatocytes, while IgG as the negative control. Process of RNA-ChIP for germ cells was as the ChIP protocol with some modifications.
Overall design Examination of γH2AX associated RNA in mouse spermatocytes
Contributor(s) Yu X, Gai X, Li Q, Xin D
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Submission date Sep 23, 2021
Last update date Sep 28, 2021
Contact name Xiaochun Yu
Organization name Westlake University
Street address 18 Shilongshan Street
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310024
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (5)
GSM5596023 IgG-IP RNA-rep1
GSM5596024 IgG-IP RNA-rep2
GSM5596025 γ-H2AX-IP-rep1
BioProject PRJNA765741
SRA SRP338498

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184711_RAW.tar 90.0 Kb (http)(custom) TAR (of TXT)
GSE184711_mmu_rRNA_45S.binned.gtf.gz 18.1 Kb (ftp)(http) GTF
GSE184711_mmu_rRNA_45S.fa.gz 4.3 Kb (ftp)(http) FA
GSE184711_mmu_rRNA_45S.gtf.gz 352 b (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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