 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 26, 2021 |
| Title |
γH2AX-associated RNA in mouse spermatocytes |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
In the testis of the mice, most spermatogonia are in pachytene stage. We have harvested spermatogonia from 3-week-old mice. As γH2AX is the marker of the XY body, we performed RNA-seq following RNA-ChIP using anti-γH2AX antibody in germ cells to report the γH2AX associated RNA in mouse spermatocytes, while IgG as the negative control. Process of RNA-ChIP for germ cells was as the ChIP protocol with some modifications.
|
| |
|
| Overall design |
Examination of γH2AX associated RNA in mouse spermatocytes
|
| |
|
| Contributor(s) |
Yu X, Gai X, Li Q, Xin D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 23, 2021 |
| Last update date |
Sep 28, 2021 |
| Contact name |
Xiaochun Yu |
| Organization name |
Westlake University
|
| Street address |
18 Shilongshan Street
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310024 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA765741 |
| SRA |
SRP338498 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184711_RAW.tar |
90.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE184711_mmu_rRNA_45S.binned.gtf.gz |
18.1 Kb |
(ftp)(http) |
GTF |
| GSE184711_mmu_rRNA_45S.fa.gz |
4.3 Kb |
(ftp)(http) |
FA |
| GSE184711_mmu_rRNA_45S.gtf.gz |
352 b |
(ftp)(http) |
GTF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...