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| Status |
Public on Mar 03, 2023 |
| Title |
Murine platelet miRNA pattern and its regulation by a MK/platelet-specific genetic ablation of Dicer. |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Purpose: To assess the platelet miRNA pattern which is regulated in a Dicer-dependent manner, we conducted a global screen of platelet miRNA expression of murine platelets from Dicerlox/lox and DicerPf4∆/Pf4∆ mice using Next-generation deep sequencing analysis.
Methods: Platelets were isolated from 10- to 12-week-old Dicerlox/lox and DicerPf4Δ/Pf4Δ mice of male sex. Total RNA including miRNA was isolated from murine platelets using the QIAsymphony RNA Kit. The sequencing library was prepared using the QIAseq miRNA Library Kit with 10 to 20 ng input. The libraries were pooled in an equimolar fashion and sequenced on a NovaSeq6000 as paired-end reads with a sequencing depth of >12 Mio clusters (i.e. read-pairs) per sample.
Results: we were able to detect a total of 427 miRNAs in these platelets, of which 81 were significantly regulated (> |0.5| logFC threshold) in a Dicer-dependent manner. The majority of miRNAs were significantly downregulated, but a smaller proportion of miRNAs was slightly, but significantly upregulated in platelets lacking Dicer.
Conclusions: this study discloses differential expression patterns of further platelet miRNAs that potentially link multiple intercellular processes and pathways implicated in several stages of the pathogenesis of myocardial I/R injury
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| Overall design |
Platelets miRNA profiling of 10- to 12-week-old Dicerlox/lox and DicerPf4Δ/Pf4Δ mice of male sex
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| Contributor(s) |
Schütte JP, Casadei N, Borst O |
| Citation(s) |
36891903 |
| |
| Submission date |
Sep 21, 2021 |
| Last update date |
Jun 05, 2023 |
| Contact name |
Oliver Borst |
| Organization name |
University Clinic Tübingen
|
| Department |
Internal Medicine III- Cardiology
|
| Lab |
AG Borst
|
| Street address |
Otfried-Müller-Street 10
|
| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA764916 |
| SRA |
SRP338060 |
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